Effects of repeated cigarette smoke extract exposure over one month on human bronchial epithelial organotypic culture

Publication date: Available online 18 August 2018Source: Toxicology ReportsAuthor(s): Shigeaki Ito, Kanae Ishimori, Shinkichi IshikawaAbstractCigarette smoke is a known risk factor for inflammatory diseases in the respiratory tract, and inflammatory exacerbation is considered pivotal to the pathogenesis of these diseases. Here, we performed two repeated exposure studies in which we exposed human bronchial epithelial tissues in an organotypic culture model to cigarette smoke extract (CSE); the first study was conducted over a four-day period to determine the suitable dose range for the extended exposure period, and the second was a one-month exposure study to elucidate the exposure-by-exposure effects in bronchial tissues. We focused on matrix metalloproteinase (MMP)-9 and -1/3 and the inflammatory cytokines interleukin (IL)-8 and growth factor related oncogene to evaluate the transition into an inflammatory state. Even at CSE doses with no or low toxicity for a single exposure, the repetition of exposure induced cumulative effects on both the inflammatory responses, specifically the IL-8 and MMPs levels, and tissue morphology. Interestingly, untreated controls initially had relatively high baseline levels of these secreted proteins; these levels gradually declined, after which they showed periodic level changes, suggesting an acclimation period may be needed for this system. These results demonstrate the usability of this system for the elucidation of sub-chronic effects in v...
Source: Toxicology Reports - Category: Toxicology Source Type: research

Related Links:

Authors: Hanewinkel R PMID: 31623003 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Zum Kommentar von Prof. Reiner Hanewinkel zum Hot Topic-Thema „Raucherentwöhnung: E-Zigarette besser als klassische Nikotinersatzpräparate?“ Pneumologie 2019; 73: 336 – 337. Pneumologie. 2019 Oct;73(10):622 Authors: Pommer P PMID: 31623002 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Schulz H, Karrasch S, Bölke G, Cyrys J, Hornberg C, Pickford R, Schneider A, Witt C, Hoffmann B PMID: 31623001 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Leserbrief zu Schulz H, Karrasch S, Bölke G et al. Atmen: Luftschadstoffe und Gesundheit – Teil I. Pneumologie 2019; 73: 288 – 305. Pneumologie. 2019 Oct;73(10):617-619 Authors: Schnitzler A PMID: 31623000 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Schöll N, Rohde GGU Abstract Pneumonia belongs to the most frequent and most deadly infectious diseases worldwide. It represents an increasing problem for the aging population. The incidence and mortality rises with every decade. The clinical presentation of pneumonia differs between elderly and younger patients. Multiple factors including functional status (self-dependency and immobilization), comorbidities, immunosenescence, nutritional status, swallowing disorders have to be accounted for. Pneumonia in the elderly has to be differentiated from nursing home acquired pneumonia. Diagnosis of pneumonia...
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Perikardpunktion – Schritt für Schritt. Pneumologie. 2019 Oct;73(10):597-604 Authors: Ferrari MW PMID: 31622998 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Raspe M, Rolling T, Leisse C, Fischer J, Lehmann C Abstract Infectious Diseases are a cross-sectional area connected to various medical disciplines and offer interested physicians multiple working opportunities. The spectrum of infectious diseases covers both out- and inpatient care as well as basic, clinical and epidemiological research. The need for infectious diseases specialists is increasing, thus career prospects are promising. Working conditions in infectious diseases are comparatively family-friendly. With this article we intend to arouse interest for working in the fascinating fields of infectious...
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Bahmer T, Wälscher J, Fisser C, Kreuter M, Karg O, Böing S, Koczulla R, Raspe M PMID: 31622996 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Lungengesundheit: Beruf als großer Risikofaktor? Pneumologie. 2019 Sep;73(9):e2 Authors: Simon A PMID: 31622984 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
20 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Trop Med Hyg These pubmed results were generated on 2019/10/19PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Source: Am J Trop Med Hyg - Category: Infectious Diseases Tags: Report Source Type: research
More News: Respiratory Medicine | Smokers | Study | Toxicology