The Research of Acellular Pancreatic Bioscaffold as a Natural 3-Dimensional Platform In Vitro

Objective The aim of the study was to investigate the biochemical and functional properties of a rat acellular pancreatic bioscaffolds (APBs). Methods Fresh pancreata from 10 rats were soaked and perfused through portal veins using Easy-Load Digital Drive peristaltic pumps. The histological structure, extracellular matrix composition, and the DNA content of the APBs were evaluated. Biocompatibility studies had also been performed. The proliferation and differentiation of AR42J pancreatic acinar cells were assessed. Results The pancreatic tissue became translucent after decellularization. There were no visible vascular endothelial cells, cellular components, or cracked cellular debris. The extracellular matrix components were not decreased after decellularization (P> 0.05); however, the DNA content was decreased significantly (P
Source: Pancreas - Category: Gastroenterology Tags: Original Articles Source Type: research