A risk assessment study of the occurrence and distribution of aflatoxigenic aspergillus flavus and aflatoxin b1 in dairy cattle feeds in a central northern state, nigeria

Publication date: Available online 16 August 2018Source: Toxicology ReportsAuthor(s): G.K. Omeiza, J. Kabir, J.K.P. Kwaga, C.N. Kwanashie, M. Mwanza, L. NgomaAbstractNigeria, being a tropical nation, characterized by favorable climatic conditions, may display high chances of feed contaminations due to aflatoxigenic Aspergillus flavus with the consequences of health risks associated with the consumption of dairy products. A cross-sectional study was conducted to examine the risks of occurrence and distribution of aflatoxigenic Aspergillus flavus (A. flavus) and aflatoxin B1 (AFB1) contamination levels based on the European Union (EU) and United States Food and Drug Administration (USFDA) set limits. Feeds (n = 144) were collected from selected conventional and traditional dairy herds; prepared and analyzed using immuno-affinity column (IAC) and high performance liquid chromatography (HPLC) respectively. Forty eight (55.8%) isolates were identified as A. flavus of the isolated Aspergilli (n = 86). Of this proportion, 12 (25.0%) were aflatoxigenic strains. An outrageous number of the tested feeds (86.8%, n = 144) were positive for AFB1 contamination. Detectable AFB1 concentrations ranged between (0.5 and 24.8) µgKg-1 and were distributed variously according to many factors of distribution. Eighty three (66.4%, n = 125) of the AFB1 contaminated feed samples showed AFB1 concentrations between 5 and ≥20 µgKg-1. ...
Source: Toxicology Reports - Category: Toxicology Source Type: research

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Publication date: Available online 12 October 2019Source: Journal of Microbiological MethodsAuthor(s): Jing Zhang, Lu Wang, Chong Luan, Guoxin Liu, Jie Liu, Yaohua ZhongAbstractFructooligosaccharides (FOS) are commonly regarded as prebiotics and used as components of functional foods. Currently, the industrial sucrose-to-FOS biotransformation is mainly carried out using the microbial-derived β-fructofuranosidases with transglycosylation activity as catalysts. Evaluation of the ability of a microorganism to produce β-fructofuranosidase is commonly conducted by measuring enzyme activity. However, the traditional me...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
Publication date: Available online 11 October 2019Source: Fungal BiologyAuthor(s): Anita Király, Csaba Hámori, Gyöngyi Gyémánt, Katalin E. Kövér, István Pócsi, Éva LeiterAbstractThe genome of Aspergillus nidulans accommodates two glycerol 3-phosphate dehydrogenase genes, gfdA and gfdB. Previous studies confirmed that GfdA is involved in the osmotic stress defence of the fungus. In this work, the physiological role of GfdB was characterized via the construction and functional characterization of the gene deletion mutant ΔgfdB. Unexpectedly, ΔgfdB ...
Source: Fungal Biology - Category: Biology Source Type: research
In this study, to show the availability of aoxA gene encoding AOX as a tool for metabolic engineering, we generated efficient oxalic acid (OA)-producers by genetic engineering of A. niger using aoxA gene. The OA-producing strain EOAH-1, generated by overexpression of the oxaloacetate hydrolase (EC 3.7.1.1; OAH) gene oahA in A. niger WU-2223L, produced 28 g/L OA from 30 g/L glucose during the 9-day cultivation period. Moreover, the strain EAOXOAH-1, generated by overexpression of both aoxA and oahA genes in strain WU-2223L, produced 28 g/L OA during the 7-day cultivation period. Strain EAOXOAH-1 sho...
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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