Correlation between adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) and drug resistance of esophageal cancer and reversal of drug resistance by artesunate.

Correlation between adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) and drug resistance of esophageal cancer and reversal of drug resistance by artesunate. Pathol Res Pract. 2018 Aug 07;: Authors: Wang L, Liu L, Chen Y, Du Y, Wang J, Liu J Abstract The present study investigated the correlation between the abnormal expression of adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) in esophageal cancer and the drug resistance of esophageal cancer, the reversal effect in drug resistance of artesunate (Art), and the mechanism underlying esophageal cancer using nude mice with subcutaneous xenograft as an animal model. The gene and protein expression of ABCG2 was detected in 80 cases of esophageal cancer, atypical dysplasia, and normal mucosa. A subcutaneous xenograft tumor mouse model was established by subcutaneous inoculation of esophageal cancer cell line Eca109/ABCG2 into BALB/c nu/nu nude mice. The reversal of drug resistance by Art in esophageal cancer was studied in vivo. The mice model was injected intraperitoneally with Art and/or doxorubicin (ADM). The animals were divided into six groups: high dose Art (50 mg/kg), low dose Art (25 mg/kg), ADM (1 mg/kg), ADM and high-dose Art, ADM and low-dose Art, and physiological saline as a control. ABCG2 protein expression and cellular ADM concentration were detected by flow cytometry. The mRNA and protein expressions of ABCG2 in esophageal cancer...
Source: Pathology, Research and Practice - Category: Pathology Authors: Tags: Pathol Res Pract Source Type: research