Sonodynamic therapy improves anti ‑tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft.

Sonodynamic therapy improves anti‑tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft. Oncol Rep. 2018 Aug 01;: Authors: Peng Y, Jia L, Wang S, Cao W, Zheng J Abstract Sonodynamic therapy (SDT) uses a combination of sonosensitizers and low‑intensity therapeutic ultrasound to destroy tumor cells. However, its effects on the tumor microenvironment, particularly on the immune state, remain unknown. The purpose of the present study was to examine the capacity and potency of the antitumor immunity induced by SDT. In the present study, sonosensitizer, 5‑aminolevulinic acid (5‑ALA), and/or ultrasound (US) were used to treat mouse B16F10 melanoma xenograft (1.0 MHz, 0.8 W/cm2, 10% duty cycle) and human umbilical vein endothelial cells (HUVECs; 0.87 MHz, 0.6 W/cm2, 60% duty cycle). Various immune cells, and proteins associated with the immunoregulation such as forkhead Box P3 (Foxp3), cytotoxic T‑lymphocyte associated protein 4 (CTLA‑4), and CD80 were detected by immunofluorescence staining and western blotting. The effect of SDT on blood vessels which were located in the central and peripheral area of tumor tissues was observed by transmission electron microscopy, immunohistochemical and immunofluorescence staining. The effect of SDT on intercellular adhesion molecule‑1 (ICAM‑1) expression on HUVECs was detected by western blotting and reverse t...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

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Non-ablative pulsed focused ultrasound (pFUS) targets non-thermal forces that activate local molecular and cellular immune responses. Optimal parameters to stimulate immunotherapeutic tumor microenvironments (TME) and responses in different tumor types remain uninvestigated. Flank B16 murine melanoma and 4T1 breast tumors received 1 MHz pFUS at 1 –8 MPa peak negative pressures (PNP) and were analyzed 24 hr post-sonication. Necrosis or hemorrhage were unaltered in both tumors, but pFUS induced DNA strand breaks in tumor cells at PNP ≥6 MPa.
Source: Ultrasound in Medicine and Biology - Category: Radiology Authors: Tags: Original Contribution Source Type: research
CONCLUSION: The ultra-wide-field scanning laser ophthalmoscopy imaging characteristics of choroidal melanoma in the Optos® system do not significantly change after proton beam radiotherapy after a mean follow-up of 2 years. PMID: 31466473 [PubMed - as supplied by publisher]
Source: European Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Eur J Ophthalmol Source Type: research
We present a patient with a CDKN2A germline mutation (p16-Leiden mutation) and mosaicism for neurofibromatosis type 2, who presented with a small asymptomatic pancreatic lesion, detected during endoscopic ultrasound screening of the pancreas. After resection, the lesion was found to be a well-differentiated pancreatic neuroendocrine tumor (PanNET). Molecular analysis of the tumor showed somatic loss of the second allele, supporting a causal relation of the PanNET to the underlying FAMMM syndrome. Recent data, showing the association between certain single-nucleotide polymorphisms in the CDKN2A gene and an increased inciden...
Source: The American Journal of Surgical Pathology - Category: Pathology Tags: Case Report Source Type: research
Authors: Lamagna B, Uccello V, Prisco F, Russo V, Lamagna F, Navas L, Mennonna G, Murino C, Meomartino L Abstract A 13-year-old Domestic Shorthair female cat, with a history of phthisis bulbi of the right eye, was referred for an acute increase in the size of the right ocular globe that occurred over a period of 1 month. Ocular examination revealed a large pink/greyish neoplastic mass involving the entire ocular surface. Ocular ultrasound and cytological examination confirmed intraocular tumor of the phthisical eye extending into the cranial space of the right orbit. The diagnosis of a primary, poorly pigmented iri...
Source: Veterinary Quarterly - Category: Veterinary Research Tags: Vet Q Source Type: research
A 57-year-old man with stage IIIB malignant melanoma of unknown primary presented for pretherapy FDG PET/CT that demonstrated metastatic left cervical lymph node with no other site of involvement. Following left neck dissection, nivolumab was initiated. Follow-up FDG PET/CT 3 months after initiation of nivolumab demonstrated extensive radiotracer-avid chest lymphadenopathy and multiple bone lesions. Ultrasound-guided endobronchial biopsy of the mediastinal lymph nodes demonstrated sarcoidosis.
Source: Clinical Nuclear Medicine - Category: Nuclear Medicine Tags: Interesting Images Source Type: research
ConclusionsLymphoscintigraphy with focused ultrasound follow-up of SNs is a reasonable management alternative to SNB in patients who are elderly and/or have substantial comorbidities.
Source: Annals of Surgical Oncology - Category: Cancer & Oncology Source Type: research
le Parrozzani Intraocular tumor diagnosis is based on clinical findings supported by additional imaging tools, such as ultrasound, optical coherence tomography and angiographic techniques, usually without the need for invasive procedures or tissue sampling. Despite improvements in the local treatment of uveal melanoma (UM), the prevention and treatment of the metastatic disease remain unsolved, and nearly 50% of patients develop liver metastasis. The current model suggests that tumor cells have already spread by the time of diagnosis, remaining dormant until there are favorable conditions. Tumor sampling procedures at ...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
CONCLUSIONS: HFUS and OCT can monitor the dynamic development of cutaneous melanoma in nude mice, and their imaging performance differs in different stages of tumor development. PMID: 31340908 [PubMed - in process]
Source: Journal of Southern Medical University - Category: Universities & Medical Training Authors: Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
Conclusion: Swept-source optical coherence tomography angiography represents a valid imaging technique to evaluate patients affected by choroidal melanomas. In our series, SS-OCT-A disclosed the intrinsic microvasculature of the tumor in all cases despite their size, location, and history of previous treatments.
Source: RETINA - Category: Opthalmology Tags: Original Study Source Type: research
We describe herein a patient with metastatic melanoma who developed epididymo‐orchitis followed by encephalitis while receiving pembrolizumab. The patient developed testicular pain and fever after his third dose of pembrolizumab; ultrasound evaluation demonstrated bilateral epididymo‐orchitis. He then developed headaches, fever, and altered mental status over the next week and was admitted to the hospital. Lumbar puncture revealed inflammatory changes consistent with meningoencephalitis; he did not improve with broad‐spectrum antibiotics, and an extensive workup for infectious etiologies, including cerebrospinal flui...
Source: The Oncologist - Category: Cancer & Oncology Authors: Tags: Melanoma and Cutaneous Malignancies Immune ‐ Related Adverse Events Source Type: research
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