Resorcinolic lipid 3-heptyl-3,4,6-trimethoxy-3H-isobenzofuran-1-one is a strategy for melanoma treatment

Publication date: 15 September 2018Source: Life Sciences, Volume 209Author(s): Stephanie Dynczuki Navarro, Lucas Roberto Pessatto, Alisson Meza, Edwin José Torres de Oliveira, Sarah Alves Auharek, Lizia Colares Vilela, Dênis Pires de Lima, Ricardo Bentes de Azevedo, Candida Aparecida Leite Kassuya, Osmar Ignacio Ayala Cáceres, Roberto da Silva Gomes, Adilson Beatriz, Rodrigo Juliano Oliveira, Marco Antonio Utrera MartinesAbstractAimsPrevious studies performed by our research group indicated that cytosporone analogues are capable of prevent or repair DNA damages. This work presents the evaluation of the activity of AMS35AA for metastatic murine melanoma cells (B16F10) in experimental model in vitro and, in pre-clinic assay of metastatic melanoma in vivo, using mice lineage C57BL/6.Main methodsIn vitro assays were performed: MTT and comet assay, flow cytometry evaluation, gene expression assay by RT-PCR, qualitative evaluation of cell death using B16F10 cells. In vivo assays: micronucleus and comet assay, splenic phagocytosis, melanoma murine model and histopathological analysis, using mice lineage C57BL/6 (n = 20).Key findingsIn vitro results performed by MTT assay showed that AMS35AA is cytotoxic for B16F10 cells (p < 0.05). Based on comet assay the genotoxicity of the IC50 was determined (95.83 μg/mL) (p < 0.05). These data were corroborated by flow cytometry analysis after the treatment with AMS35AA, which indicates the cellular death by apoptosis ...
Source: Life Sciences - Category: Biology Source Type: research