Gel-based proteomics in disease research: Is it still valuable?1

Publication date: Available online 15 August 2018Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Yong-In Kim, Je-Yoel ChoAbstractGel electrophoresis has been the primary method in proteomics. In the early era of proteomics, gel electrophoresis was a dominant technique of sample preparation for mass spectrometry (MS) analysis. Particularly, two-dimensional electrophoresis (2-DE) provided high-resolution proteome separation, and was regarded as the standard methodology for the separation of wide-range proteomes. However, gel electrophoresis turned downwards due to the progress of other separations including liquid chromatography and ionization techniques, resulting gel-free proteomics finally becoming dominant players at present. There are numerous advantages in gel-free approach in aspects of current trends of disease research. Although, gel-based approaches are still advanced, it seems that gel electrophoresis will not be disappeared. The unique features of gel electrophoresis can be complementary for gel-free and it is suitable for the new wave of top-down proteomics.

Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - August 15, 2018 Category: Biochemistry Source Type: research