Laccase-Catalyzed Heterocoupling of Dihydroquercetin and p -Aminobenzoic Acid: Effect of the Reaction Product on Cultured Cells

AbstractDerivatization of the natural flavonoid dihydroquercetin withp-aminobenzoic acid was carried out in an ethyl acetate/citric buffer biphasic system using laccase from the fungusTrametes hirsuta. The main reaction product yield was ~68 mol %. The product was characterized by1H NMR,13C NMR, and liquid chromatography-mass spectroscopy, and its structure was elucidated. The reaction product affected viability of cultured human rhabdomyosarcoma cells (RD cell line) in a dose-dependent manner and, therefore, can be of interest to pharmaceutical industry.
Source: Biochemistry (Moscow) - Category: Biochemistry Source Type: research