Seed germination and biochemical profile of Citrus reticulata (Kinnow) exposed to green synthesised silver nanoparticles

In this study, effects of the synthesised AgNPs were also evaluated on nucellus tissues germination frequency and biochemical parameters of plant tissues. Nucellus tissues of Citrus reticulata were inoculated on MS medium supplemented with 10, 20, 30 and 40 μg/ml suspension of the synthesised AgNPs. Green synthesised AgNPs enhanced the in vitro germination because of low toxicity and nonfriendly issues. Significant results were obtained for germination parameters i.e. root and shoot length and seedling vigour index in response to 30 μg/ml suspension of green synthesised AgNPs. The 30 μ/ml suspension of AgNPs also enhanced antioxidant activity (41%) and SOD activity (0.36 nM/min/mg FW) while total phenolic content (4.7 μg/mg FW) and total flavonoid content (1.1 μg/mg FW) was significantly high when MS medium was fortified with 40 μg/ml suspension of the synthesised AgNPs. The content of total protein was significant (558 μg/BSA Eq/mg FW) in control plantlets as compared to the other treatments.
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research