Integration-free reprogramming of human umbilical arterial endothelial cells into induced pluripotent stem cells IHSTMi001-A

Publication date: Available online 12 August 2018Source: Stem Cell ResearchAuthor(s): Huilin Li, Haiyun Pei, Jiqin Nie, Mingyi Qu, Zeng Fan, Yali Jia, Lijuan He, Xue Nan, Wen Yue, Xuetao PeiAbstractPrimary arterial endothelial cell (AEC) is an attractive source of tissue-engineered blood vessels for therapeutic transplantation in vascular disease. However, scarcity of donor tissue, inability of proliferation and undergo de-differentiation in culture remain major obstacles. We derived a stable induced pluripotent stem cell (iPSC) line possessed all the characteristics of pluripotent state from human umbilical arterial endothelial cells by transduction of four human transcription factors (Oct4, Sox2, Klf4, and c-Myc) using sendai virus vectors. It will likely facilitate to lineage differentiate and generate sufficient AECs for clinical use in cardiovascular disease based on epigenetic memory of the tissue of origin.
Source: Stem Cell Research - Category: Stem Cells Source Type: research