Characterization of phytochemical components of Ferula halophila extracts using HPLC-MS/MS and their pharmacological potentials: A multi-functional insight

Publication date: Available online 10 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Gokhan Zengin, Ahmet Uysal, Alina Diuzheva, Erdogan Gunes, József Jekő, Zoltán Cziáky, Carene Marie Nancy Picot-Allain, Mohamad Fawzi MahomoodallyAbstractThe inhibitory action of F. halophila extracts (acetone, chloroform, and methanol) against key enzymes linked to diabetes (α-amylase, α-glucosidase), cognitive enhancing functions (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE)), and hyperpigmentation (tyrosinase) was assessed. The mutagenic/antimutagenic activities were assessed and the phytochemical profile established by HPLC-MS/MS. The acetone extract showed the highest phenolic (55.22 mg GAE/g extract) and flavonoid (34.52 mg RE/g extract) contents. The chloroform extract was a potent inhibitor of cholinesterases (4.86 and 6.13 mg GALAE/g extract, against AChE and BChE, respectively). Cinnamic acid derivatives (methyl cinnamate, ferulic acid, methoxycinnamic acid isomer) were identified in the chloroform extract. Methanol extract showed potent inhibitory action against tyrosinase (137.63 mg KAE/g extract) and glucosidase (43.02 mmol ACAE/g extract). The chloroform extract (32.07 mg EDTAE/g extract) showed potent metal chelating potential. The neuroprotective action of the chloroform extract might be attributed to the metal chelating action coupled by the cholinesteras...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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In this study, we use a high-density S. Typhi transposon library in hu-SRC-SCID mice to identify virulence loci using transposon-directed insertion site sequencing (TraDIS). Vi capsule, lipopolysaccharide (LPS), and aromatic amino acid biosynthesis were essential for virulence, along with the siderophore salmochelin. However, in contrast to the murine S. Typhimurium model, neither the PhoPQ two-component system nor the SPI-2 pathogenicity island was required for lethal S. Typhi infection, nor was the CdtB typhoid toxin. These observations highlight major differences in the pathogenesis of typhoid and non-typhoidal Salmonel...
Source: Cell Host and Microbe - Category: Microbiology Authors: Tags: Cell Host Microbe Source Type: research
CONCLUSION: B. vulgaris is a potential candidate for the treatment of several health disorders associated with infectious diseases and diabetes. PMID: 31203800 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - Category: Biotechnology Authors: Tags: Curr Pharm Biotechnol Source Type: research
Salmonella infection leads to severe intestinal inflammation and increased CD4+FoxP3+ Treg cells in streptozotocin‑induced hyperglycemic mice. Mol Med Rep. 2019 Apr 25;: Authors: Zhang S, Wang M, Wang X, Li H, Tang H, Li X Abstract Hyperglycemia promotes the growth and reproduction of bacteria, thereby increasing the probability of infection, which also causes rebound hyperglycemia. Therefore, the interactions of infection and hyperglycemia lead to the progression and deterioration of these diseases. Type 1 diabetes mellitus (T1DM) is an autoimmune disease. Studies have shown that regulatory T&n...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Alessandro Poggi1*, Roberto Benelli2, Roberta Venè1, Delfina Costa1, Nicoletta Ferrari1, Francesca Tosetti1 and Maria Raffaella Zocchi3 1Molecular Oncology and Angiogenesis Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy 2Immunology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy 3Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy It is well established that natural killer (NK) cells are involved in both innate and adaptive immunity. Indeed, they can recognize molecules induced at the cell surface by stress signals ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Gulcin Tezcan1, Ekaterina V. Martynova1, Zarema E. Gilazieva1, Alan McIntyre2, Albert A. Rizvanov1 and Svetlana F. Khaiboullina1,3* 1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia 2Centre for Cancer Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, United Kingdom 3Department of Microbiology and Immunology, University of Nevada, Reno, Reno, NV, United States Inflammation has a crucial role in protection against various pathogens. The inflammasome is an intracellular multiprotein signaling complex that is linked to pathogen sensing and...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Conclusions and Perspectives It is interesting that watershed events, such as the loss of Neu5Gc from the glycocalyx of human cells have occurred numerous times in many mammalian and other vertebrate species. These cases of convergent evolution represent precious opportunities for increased understanding of evolutionary processes. In some respects, Neu5Gc is an ideal self-molecule as it is “private” to vertebrates and, based on current data, has yet to be successfully mimicked by microbes. Against the background of this benefit, the loss of Neu5Gc appears paradoxical and may implicate strong selective regimes,...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, we unraveled the secretome of G. adiacens, an oral bacterium well-documented in infective endocarditis, but also recently shown to be involved in oral infections. Importantly, the secretome of G. adiacens comprised of a large number of putative virulence factors. Of particular importance is the finding that the G. adiacens secretome comprised of a number of “moonlighting” proteins, which in other species are shown to enhance bacterial colonization and virulence through their multifunctional roles (Pavkova et al., 2017; Graf et al., 2019). Thus, our results provide a basis for investigating the ro...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
We describe a case of Salmonella neck abscess in a diabetic patient. An 18 year old diabetic male was admitted with increasing left sided neck pain and swelling approximately four weeks after gastrointestinal illness. Imaging revealed a left sided neck abscess. Surgical drainage was undertaken. Cultures grew non-typhoid Salmonella species. He was treated with intravenous antibiotics and did well clinically. Salmonella infection should be considered in the differential diagnosis of patients with immunocompromising conditions presenting with neck abscess of unclear etiology.
Source: IDCases - Category: Infectious Diseases Source Type: research
In conclusion, our study might provide evidence that GQD treats diarrhea maybe involved in modulating gut microbiota and increasing SCFA levels. Introduction Diarrheal diseases cause approximately 1.7 billion new infections and 0.76 million deaths in children of under 5 years of age annually and are thus the second most common cause of mortality in young children in developing countries (Kotloff et al., 2012, 2013). Outbreaks of such diseases are usually related to infection by common causative agents, mainly rotavirus, Salmonella enterica, and diarrheagenic Escherichia coli (Hodges and Gill, 2010; Kolenda et al., ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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