Intraluminal Pressure Triggers Myogenic Response via Activation of Calcium Spark and Calcium-Activated Chloride Channel in Rat Renal Afferent Arteriole.

Intraluminal Pressure Triggers Myogenic Response via Activation of Calcium Spark and Calcium-Activated Chloride Channel in Rat Renal Afferent Arteriole. Am J Physiol Renal Physiol. 2018 Aug 08;: Authors: Yip KP, Balasubramanian L, Kan C, Wang L, Liu R, Ribeiro-Silva L, Sham JSK Abstract Myogenic contraction of renal arterioles is an important regulatory mechanism for renal blood flow autoregulation. We have previously demonstrated that integrin-mediated mechanical force increases the occurrence of Ca2+ sparks in freshly isolated renal vascular smooth muscle cells (VSMCs). To further test whether generation of Ca2+ sparks is a downstream signal of mechanotransduction in pressure induced myogenic constriction, the relationship between Ca2+ spark frequency and transmural perfusion pressure was investigated in intact VSMCs of pressurized rat afferent arterioles. Spontaneous Ca2+ sparks were found in VSMCs when afferent arterioles were perfused at 80 mmHg. The spark frequency was significantly increased when perfusion pressure was increased to 120 mmHg. Similar increase of spark frequency was also observed in arterioles stimulated with β1-integrin activating antibody. Moreover, spark frequency was significantly higher in arterioles of spontaneous hypertensive rats (SHR) at 80 and 120 mmHg. Spontaneous membrane current recorded with whole-cell perforated-patch in renal VSMCs showed predominant activity of spontaneous transient inward curr...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research
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