Performance of an in-house real-time PCR assay for detecting Cytomegalovirus infection among transplant patients from a tertiary care centre

Conclusion: The in-house viral load assay had a better correlation with clinical findings compared to the commercial assay. Quality assessment of these assays was done by the United Kingdom National External Quality Assessment Scheme (UKNEQAS), an external proficiency testing programme, and by the National Institute for Biological Standard and Control (NIBSC) standard. For UKNEQAS and NIBSC standards, the bias between the assays was 0.73 log10and 0.85 log10, respectively. This difference is well within the acceptable range already reported in the literature.

Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Santhosh Kumar Duraisamy Shoba Mammen Sasi Kumar Reddy Lakshminarayan Susan Verghese Mahesh Moorthy Biju George Rajesh Kannangai Santosh Varghese Alok Srivastava Asha Mary Abraham Source Type: research