Fabrication of Dihydroxyflavone-conjugated Hyaluronic Acid Nanogels for Targeted Antitumoral Effect
Publication date: Available online 6 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yu Ri Choi, Hyun-Jong Kim, Guk Young Ahn, Min Jeong Lee, Ju Ri Park, Dae-Ryong Jun, Tae-Kyoung Ryu, Joo Woong Park, Eunji Shin, Sung-Wook ChoiAbstractWe prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60 % for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research