GAPDH with NAD+-binding site mutation competitively inhibits the wild-type and affects glucose metabolism in cancer

ConclusionMutant-GAPDH affects the enzymatic function of cellular-GAPDH and disrupts energy metabolism.General significanceOur findings demonstrate that a minimal mutation at the NAD+-binding site is sufficient to generate a competitive but dysfunctional GAPDH, and its ectopic expression inhibits the wild-type to disrupt glycolysis.Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) General Subjects - Category: Biochemistry Source Type: research