An attempt to characterize the human Chorionic Gonadotropin protein by reversed phase liquid chromatography coupled with high-resolution mass spectrometry at the intact level

Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julien Camperi, Audrey Combes, Jean Guibourdenche, Davy Guillarme, Valerie Pichon, Thierry Fournier, Nathalie DelaunayAbstractFor the first time, the human Chorionic Gonadotropin (hCG) hormone at the intact level was characterized by reversed phase liquid chromatography (RPLC) coupled with high resolution mass spectrometry (HRMS). This heterodimeric protein is specific to human pregnancy, consists in an α and a β subunit, so-called hCGα and hCGβ, respectively, and has 8 glycosylation sites leading to a high number of isoforms. First, the LC method was optimized to separate the largest number of isoforms and also to facilitate the MS ionization process and data treatment. The initial mobile phase composition, slope of the gradient, and column temperature were appropriately selected to maximize the number of separated isoforms. Moreover, the MS detection parameters were adjusted to i) promote the efficient transfer of the heaviest ions, ii) avoid or limit the fragmentation of the ions and iii) improve the sensitivity. The repeatability of the final method in terms of retention times and peak areas was assessed. The method was next used to characterize two hCG-based drugs: Ovitrelle® (a recombinant hCG, r-hCG) and Pregnyl® (hCG isolated from urine of pregnant women, u-hCG). After the deconvolution step, the analytical method did not al...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: September 2019Source: Advances in Chronic Kidney Disease, Volume 26, Issue 5Author(s): Sharon Maynard, Grace Guerrier, Margaret DuffyPregnancy is an altered immunologic state in which hormonal changes impact the immune system to enable maternal tolerance of the fetus. These hormonal and immunologic changes may affect disease activity in systemic lupus erythematosus. Conversely, lupus nephritis and its complications may adversely impact pregnancy. Systemic lupus erythematosus increases the risk of pre-eclampsia and its complications, including preterm birth and intrauterine growth restriction. Comorbiditie...
Source: Advances in Chronic Kidney Disease - Category: Urology & Nephrology Source Type: research
BACKGROUND: Violence and other adversities commonly co-occur, yet are usually investigated individually. The primary objectives of this paper are to investigate: (i) the relationship between maternal exposure to violence (including childhood abuse and inti...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
Publication date: November 2019Source: Journal of Comparative Pathology, Volume 173Author(s): J.W. Harvey, K.E. Harr, D. Murphy, M.T. Walsh, M. de Wit, C.J. Deutsch, R.K. BondeSummarySerum iron concentration is usually decreased in true iron deficiency and with inflammatory disease in man and domestic animals. Serum total iron binding capacity (TIBC) may be increased in true iron deficiency and decreased with inflammatory disease. This prospective study was designed to measure serum iron analytes in healthy free-ranging and housed Florida manatees (Trichechus manatus latirostris) of both sexes and various ages and to evalu...
Source: Journal of Comparative Pathology - Category: Pathology Source Type: research
Publication date: Available online 14 November 2019Source: Pregnancy HypertensionAuthor(s): Wibke G. Janzarik, Jenny Jacob, Evi Katagis, Filiz Markfeld-Erol, Linda Sommerlade, Matthias Wuttke, Matthias Reinhard
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - Category: OBGYN Source Type: research
Publication date: Available online 14 November 2019Source: Best Practice &Research Clinical Obstetrics &GynaecologyAuthor(s): Emily C. SomersAbstractAutoimmune diseases (AID) are more prevalent in women than men, and pregnancy-related factors such as hormonal modulation and fetal microchimerism may influence future risk of maternal AID. For women with AID, optimizing reproductive health requires a continuum of multidisciplinary care that initiates well before the desire for pregnancy is articulated. Family planning is essential so that pregnancy can be timed when disease is stable and to allow for appropriate medic...
Source: Best Practice and Research Clinical Obstetrics and Gynaecology - Category: OBGYN Source Type: research
Ann Kihii (25) spends time with other young women from poor communities in Nairobi and use embroidery to create images that tell a story about the daily challenges they face. They also get a chance to discuss the issues among themselves in a safe space. Credit: Mantoe Phakathi / IPSBy Mantoe PhakathiNAIROBI, Kenya, Nov 14 2019 (IPS) While women find it hard to talk about their painful experiences, some have found a way of expressing themselves through art. Women, trained as artists, from Nairobi’s informal settlements Kibera and Kangemi, have produced a beautiful quilt that tells stories about their daily challenges....
Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Africa Arts Conferences Education Featured Gender Gender Violence Headlines Health Human Rights TerraViva United Nations Women's Health ICPD25 Source Type: news
Conditions:   Rheumatoid Arthritis;   Psoriatic Arthritis;   Crohn's Disease;   Axial Spondyloarthritis;   Plaque Psoriasis Intervention:   Drug: Pharmacokinetics of certolizumab pegol Sponsor:   UCB Biopharma S.P.R.L. Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
ConclusionIt is known that sphingolipids serve as modulators of liver regeneration. We assume these substances could be potential markers for detecting early onsets of intrahepatic cholestasis of pregnancy.
Source: Prostaglandins and Other Lipid Mediators - Category: Lipidology Source Type: research
ICPD25 Youth delegates: Michele Simon (left) and Botho Mahlunge. Credit: Joyce Chimbi / IPSBy Joyce ChimbiNAIROBI, Kenya, Nov 14 2019 (IPS) Every day in developing countries it is estimated that 20,000 girls under the age of 18 give birth. This amounts to 7.3 million births a year. Complications from pregnancy and childbirth are still the leading cause of death among adolescent girls, according to the United Nations Population Fund (UNFPA) statistics. Let us be heard Born long after the Cairo Promise, the 18-year-old Michelle Simon and the 19-year-old Botho Mahlunge both youth representatives from Botswana, lament that ye...
Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Africa Conferences Education Featured Gender Gender Violence Global Headlines Health Human Rights TerraViva United Nations Women's Health ICPD25 Source Type: news
State policies that treat pregnant women like criminals if they use illicit substances are linked with higher rates of babies being born with opioid withdrawal.
Source: L.A. Times - Health - Category: Consumer Health News Authors: Source Type: news
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