Multiresidue determination of 21 pharmaceuticals in crayfish (Procambarus Clarkii) using enzymatic microwave-assisted liquid extraction and ultrahigh-performance liquid chromatography - triple quadrupole mass spectrometry analysis

Publication date: Available online 2 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Julia Kazakova, Rut Fernández-Torres, María Ramos-Payán, Miguel Ángel Bello-LópezAbstractIn this paper, a multiresidue enzymatic-microwave assisted extraction prior to ultrahigh performance liquid chromatography and triple quadrupole mass spectrometry analysis has been developed for the determination of 21 pharmaceuticals in crayfish (Procambarus Clarkii) samples. The analysed compounds corresponding to 6 therapeutic families were: fluoroquinolones (ciprofloxacin, danofloxacin, enrofloxacin, flumequine, gatifloxacin, grepafloxacin, marbofloxacin and norfloxacin); tetracyclines (chlortetracycline and oxytetracycline); sulphonamides (sulfamethoxazole, sulfadiazine, sulfamethazine, sulfamerazine); penicillins (amoxicillin); anfenicols (chloramphenicol, thiamphenicol and florfenicol); non-steroidal anti-inflammatory drugs (ibuprofen and salicylic acid) and trimethoprim an antibiotic that is frequently co-administered with sulfamethoxazole. The main factors affecting the extraction efficiency were optimized for 0.5 g of lyophilized tissue. The enzymatic microwave extraction was carried out using an extraction time of 5 min with 5 mL of an acetonitrile: water (1:1, v/v) mixture, 50 µL of Proteinase-K solution and 5 µL of formic acid at 50 W. After centrifugation, the liquid extract was evaporated and the residue was reconstituted with 1 mL of 0.1%...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research