Interaction of calmodulin with poly(acrylic acid) brushes: effects of high pressure, pH-value and ligand binding

Publication date: Available online 31 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Artem Levin, Claus CzeslikAbstractPoly(acrylic acid) (PAA) brushes are well-known to interact with proteins in an ionic strength-dependent way. Moreover, they provide a native-like environment that largely maintains the secondary structure and biological activity of adsorbed proteins. Recently, it has been shown that the application of high pressure can lead to a reduced protein adsorption at a PAA brush in the case of a positively charged protein. Here, we analyze the effect of pressure on the interactions between a protein and a PAA brush in more detail. We use calmodulin as model protein that has a negative net charge at neutral pH-value and determine the degree of adsorption at a planar PAA brush applying total internal reflection fluorescence (TIRF) spectroscopy. Remarkably, the degree of calmodulin adsorption at a PAA brush is increasing with increasing pressure, when the protein is negatively charged. However, at low pH-value, where calmodulin is positively charged, high pressure leads to a partial desorption of the protein. Moreover, in the presence of trifluoperazine, which binds to calmodulin as a ligand, the pressure effect is diminished. The results of this study indicate that protein adsorption at a PAA brush at the “wrong” side of the isoelectric point, i.e. under net electrostatic repulsion, can involve a volume reduction that is favored under high pressu...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research