Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis

Publication date: Available online 29 July 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Michał Gondek, Justyna Bień, Zygmunt NowakowskiABSTRACTThe aim of the present study was to evaluate the detection of trichinellosis using ELISA, and to determine the degree of variation of IgG antibodies against excretory-secretory (ES) antigens of T. spiralis muscle larvae. Ten young Polish Large White native breed pigs were experimentally inoculated with a low dose of 300 invasive Trichinella spiralis (T. spiralis) muscle larvae (ML). Pig sera were collected at 7 and 9 days prior to the experimental infection with T. spiralis and at 9, 14, 20, 23, 25, 27, 30, 33, 37, 41 and 46 days post-infection (d.p.i.). Western blotting was used as a follow-up test to detect anti-T. spiralis IgG and confirm the results provided by ELISA. The molecular weight of the E-S antigenic proteins of T. spiralis muscle larvae reacting with the sera from the infected pigs was determined. Finally, the intensity of T. spiralis infection (lpg) was determined as an average value of 16 muscles taken from each pig. The E-S antigen of T. spiralis muscle larvae in the in-house ELISA and immunoblotting assays did not demonstrate any cross reaction with non-infected pig sera and sera from pigs naturally infected with Oesophagostomum spp. The ELISA assay did not recognize trichinellosis in pigs until 27 days after the T. spiralis infection. The anti-Trichinella IgG antibodies were first detected on day...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research