Altered Formation of the Iron Oxide Nanoparticle-Biocorona due to Individual Variability and Exercise

Publication date: Available online 29 July 2018Source: Environmental Toxicology and PharmacologyAuthor(s): Lisa M. Kobos, Sherleen Xue-Fu Adamson, Sheelagh Evans, Timothy Gavin, Jonathan H. ShannahanAbstractNanoparticles (NPs), introduced into a biological environment, accumulate a coating of biomolecules or biocorona (BC). Although the BC has toxicological and pharmacological consequences, the effects of inter-individual variability and exercise on NP-BC formation are unknown. We hypothesized that NPs incubated in plasma form distinct BCs between individuals, and exercise causes additional intra-individual alterations. 20 nm iron oxide (Fe3O4) NPs were incubated in pre- or post-exercise plasma ex vivo, and proteomics was utilized to evaluate BC components. Analysis demonstrated distinct BC formation between individuals, while exercise was found to enhance NP-BC complexity. Abundance differences of NP-BC proteins were determined between individuals and resulting from exercise. Differential human macrophage response was identified due to NP-BC variability. These findings demonstrate that individuals form unique BCs and that exercise influences NP-biomolecule interactions. An understanding of NP-biomolecule interactions is necessary for elucidation of mechanisms responsible for variations in human responses to NP exposures and/or nano-based therapies.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research