Insights of Leishmania (Viannia) braziliensis infection in golden hamster (Mesocricetus auratus) intestine

Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Amanda Gubert Alves dos Santos, Lainy Leiny de Lima, Camila Alves Mota, Marcelo Biondaro Gois, Andrea Claudia Bekner Silva Fernandes, Thaís Gomes Verzignassi Silveira, Debora de Mello Gonçales Sant’Ana, Gessilda de Alcântara Nogueira de MeloAbstractAimThe present study compared and evaluated morphological and quantitative alterations in the ileum of hamsters infected by two L. (V.) braziliensis strains isolated from patients with different lesion aspects and treatment responses.Main methodsHamsters were infected in the left hindpaw with a suspension of promastigotes (2 × 107/100 μl) of two different strains of L. (V.) braziliensis. After 90 or 120 days, the animals were euthanized. Samples of the ileum and mesenteric lymph node were collected for histological examination and quantitative polymerase chain reaction.Key findingsAll infected animals developed similar profile of paw lesions. In peripheral blood there was an increase in the number of mononuclear cells which contributed to elevated global leukocytes count. Increases in the width and height of villi and width and depth of crypts were observed. The thickness of the muscular layers, submucosa, and intestinal wall also increased. Histopathological alterations were observed, including inflammatory infiltrate in crypts and a large number of immune cells in the lamina propria, submucosa, and muscular layer. Immune cells w...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research