Cytomegalovirus Infection: Mouse Model.

Cytomegalovirus Infection: Mouse Model. Curr Protoc Immunol. 2018 Jul 25;:e51 Authors: Brizić I, Lisnić B, Brune W, Hengel H, Jonjić S Abstract This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc. PMID: 30044539 [PubMed - as supplied by publisher]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research