Development and validation of a rapid ultra-high performance liquid chromatography diode array detector method for Verbena officinalis L

This study presents a fast and validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the simultaneous determination of the major compounds in Verbena officinalis L. (Verbenaceae), a medicinal plant listed in the European, British, and, Chinese Pharmacopoeias. In order to get reference substances, nine compounds, belonging to iridoids, flavonoids, and phenylpropanoid glycosides, were isolated from the herb extract. Two of them, namely cistanoside D and leucosceptoside A, were found in this plant species for the first time. Chromatographic separation was achieved in less than 7 min on a Kinete x 1.7 u XB-C18 (50 x 2.10 mm) column by using a solvent gradient of water-acetonitrile modified with 0.1% formic acid. Method validation confirmed the assays sensitivity, linearity (R2 ≥ 0.997), precision (intraday precision ≤ 1.71%; interday precision ≤ 1.46%) and accuracy (recovery rates between 93.9% and 108.8%) for the quantitative analysis of the eight selected marker compounds. Identity and peak purity of the analytes was confirmed by coupling the UHPLC instrument to a quadrupole time-of-flight mass spectrometer via an electrospray ionization interface (ESI-QTOF-MS) operating in the negative ionization mode.Finally, the applicability of the developed UHPLC-DAD method was successfully proven for the sensitive quantitation of the major compounds in Verbena herb extracts, thereby prov...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 16 November 2019Source: Journal of Health EconomicsAuthor(s): Dimitris Christelis, Dimitris Georgarakos, Anna Sanz-de-GaldeanoAbstractEconomic theory predicts that a reduction in background risk should induce financial risk-taking, particularly for individuals with low stock market participation costs. Hence, health insurance coverage could affect financial risk-taking by offsetting health-related background risk. We use a regression discontinuity design to examine whether Medicare eligibility at age 65 increases stockholding in the US and find that it does so for those with college educa...
Source: Journal of Health Economics - Category: Health Management Source Type: research
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Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 17 November 2019Source: European UrologyAuthor(s): Tom Marcelissen, Kevin Rademakers
Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: European Urology - Category: Urology & Nephrology Source Type: research
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Source: Research in Autism Spectrum Disorders - Category: Psychiatry Source Type: research
ConclusionThis meta-analysis suggested a significant association between MTHFR gene polymorphism (C677T and A1298C) and ASD risk.
Source: Research in Autism Spectrum Disorders - Category: Psychiatry Source Type: research
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Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
ConclusionsTattooing of axillary LNs is safe and easily performed. Tattooing was helpful in identifying the marked LN in the majority of cases. This technique helps to ensure that metastatic LNs are identified and removed at surgery after NAT.
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
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