Antifungal susceptibility of Aspergillus spp., Scedosporium spp. and Exophiala spp. isolated from patients with cystic fibrosis

Background: Moulds are frequently recovered from respiratory samples of cystic fibrosis (CF) patients. During the last decade, antifungal resistance in non-Candida species is increasingly reported. Azole resistance in Aspergillus fumigatus is a well-known problem and it was firstly reported in 1997 in three itraconazole-resistant clinical isolates. Since then, the main azole resistance mechanism reported are mutations at the CYP51A gene which encodes the azole drug target, the 14- α-sterol demethylase.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: UMP. 494 Source Type: research

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Aim: To investigate the role of different types of bronchial infection and respiratory symptoms upon lung function decline.Methods: 89 children and adults with CF (mean (SD) age: 11.71 (6) years) performed 1340 serial Multiple Breath Washout (MBW) tests and 980 spirometries over a 5-year period. Respiratory symptoms (cough, sputum, nasal congestion) and Pulmonary Exacerbations (PEx) were recorded. Pseudomonas Aeruginosa, Staphylococcus aureus infection, as well as aspergillus, candida, Achromobacter, Acinetobacter, Serratia and Stenotrophomonas Maltophilia isolated from cough swabs/ sputum cultures were recorded.Results: M...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Cystic fibrosis Source Type: research
In conclusion, circulating MDSCs are measurable, functional and have a G-MDSC phenotype in lung transplant patients. Their frequency is increased in stable patients, decreased during post-transplant complications, and related to level of immunosuppression. This study may pave the way for further investigations of MDSC in the context of lung transplantation. Introduction From a transplant immunological point of view, graft acceptance is the fundamental element in allograft survival. Graft acceptance is realized by blocking the immune system with immunosuppression preventing host immune cells to recognized and attack...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In this study they also showed PTX3 localized in NETs formed after neutrophil activation (5). Proteomics analysis revealed that PTX3 forms complexes with two anti-microbial proteins [azurocidin (AZU1) and myeloperoxidase (MPO)] associated to NETs (30). More recently, PTX3 localization in NETs has been confirmed, and the colocalization with AZU1 and MPO has been defined more accurately (31). Further investigation will be needed to understand the involvement of PTX3 interaction with AZU1 and MPO in their antibacterial role during NET formation. Regulation of Complement Activation PTX3 interaction with microorganisms is not...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Raquel Ferrer-Espada1,2, Xiaojing Liu1,2, Xueping Sharon Goh1,2 and Tianhong Dai1,2* 1Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States 2Vaccine &Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States Polymicrobial biofilms, in which mixed microbial species are present, play a significant role in persistent infections. Furthermore, polymicrobial biofilms promote antibiotic resistance by allowing interspecies transfer of antibiotic resistance genes. In the present study, we investigated the effec...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Interactions in the airway ecology of cystic fibrosis may alter organism persistence and clinical outcomes. Better understanding of such interactions could guide clinical decisions. We used generalized estimating equations to fit logistic regression models to longitudinal 2-year patient cohorts in the Cystic Fibrosis Foundation Patient Registry, 2003 to 2011, in order to study associations between the airway organisms present in each calendar year and their presence in the subsequent year. Models were adjusted for clinical characteristics and multiple observations per patient. Adjusted models were tested for sensitivity to...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Epidemiology Source Type: research
Case 1: A 20-years old woman with cystic fibrosis (CF), deltaF508 homozygote, predicted 35% FEV1 was treated with inhalative antibiotics due to colonisation with Pseudomonas aeruginosa (PA). An allergic bronchopulmonary aspergillosis has previously been treated, with no evidence of reactivation. After severe exacerbations and a drop of FEV1 to 17%, she stabilized with steroids and IV-antibiotics. Despite a reduction of Pseudomonas, a low FEV1 persisted. Yeast grew in repeated sputum cultures, but could not be identified by standard methods. ITS-sequencing revealed the presence of Blastobotrys raffinosifermentans. Adequate ...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Cystic Fibrosis Source Type: research
Conclusion: Our findings represent the first characterisation of the Asian pulmonary microbiome in bronchiectasis, including analysis of the bacterial and fungal microbiota. The direct and mechanistic implications of these findings on clinical disease, in comparison to Caucasian cohorts, require further investigation.Funding: This research is supported by the Singapore Ministry of Health’s National Medical Research Council under its Transition Award (NMRC/TA/0048/2016) (S.H.C).
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Respiratory Infections Source Type: research
Abstract Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage. The inhibition of biofilm was reverse...
Source: Microbiology - Category: Microbiology Authors: Tags: Microbiology Source Type: research
Abstract BackgroundProspective studies to determine associated risk factors and related outcomes for pulmonary fungal infection (PFI) after pediatric lung transplant (PLT) are lacking. MethodsNIH‐sponsored Clinical Trials in Organ Transplantation in Children enrolled PLT candidates, collecting data prospectively for 2 years post‐transplant. Demographics, signs/symptoms, radiology, pathology and microbiology were collected. Analyses evaluated for PFI‐related risks and outcomes. ResultsIn 59 PLT, pre‐transplant fungal colonization occurred in 6 donors and 15 recipients. Cystic fibrosis (CF) was associated with pre‐...
Source: Clinical Transplantation - Category: Transplant Surgery Authors: Tags: Original Article Source Type: research
Abstract Lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (CF). N-chlorotaurine (NCT), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. The aim of this study was to investigate NCT on its bactericidal and fungicidal activity in vitro in artificial sputum medium (ASM) mimicking the composition of cystic fibrosis mucus.The medium was inoculated with bacteria (Staphylococcus aureus including some MRSA strains, Pseudomonas aeruginosa, Escherichia coli) or spores of fungi (Aspergillus fumigatus, Aspergi...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
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