Evaluation of Modified Two-Tiered Testing Algorithms for Lyme Disease Laboratory Diagnosis Using Well-Characterized Serum Samples Immunoassays

Standard two-tiered testing (STTT) is the recommended algorithm for laboratory diagnosis of Lyme disease (LD). Several limitations are associated with STTT that include low sensitivity in the early stages of disease, as well as technical complexity and subjectivity associated with second-tier immunoblotting; therefore, modified two-tiered testing (MTTT) algorithms that utilize two sequential first-tier tests and eliminate immunoblotting have been evaluated. Recently, a novel MTTT that uses a VlsE chemiluminescence immunoassay followed by a C6 enzyme immunoassay has been proposed. The purpose of this study was to evaluate the performance of the VlsE/C6 MTTT using well-characterized serum samples. Serum samples from the CDC Lyme Serum Repository were tested using three MTTTs, VlsE/C6, whole-cell sonicate (WCS)/C6, and WCS/VlsE, and three STTTs (immunoblotting preceded by three different first-tier assays: VlsE, C6, and WCS). Significant differences were not observed between the results of the MTTTs assessed; however, the VlsE/C6 MTTT resulted in the highest specificity (100%) when other diseases were tested and the lowest sensitivity (75%) for LD samples. Significant differences were present between the results for various MTTTs and STTTs evaluated. Specifically, all MTTTs resulted in higher sensitivities than the STTTs for all LD groups combined and were significantly more accurate (i.e., higher proportion of correct classifications) for this group, with the exception of the W...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research

Related Links:

(Harvard Medical School) A $5 million donation to Harvard Medical School will propel Lyme disease research, education
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Publication date: Available online 1 July 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Saied Reza Naddaf, Ahmad Mahmoudi, Ahmad Ghasemi, Mahdi Rohani, Ali Mohammadi, Seyyed Payman Ziapour, Amir Hesam Nemati, Ehsan Mostafavi
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Pathogen specific antibody responses need to be tightly regulated to generate protective but limit self-reactive immune responses. While loss of humoral tolerance has been associated with microbial infections, the pathways involved in balancing protective versus autoreactive antibody responses in humans are incompletely understood. Studies in classical mouse model systems have provided evidence that balancing of immune responses through inhibitory receptors is an important quality control checkpoint. Genetic differences between inbred mouse models and the outbred human population and allelic receptor variants not present i...
Source: eLife - Category: Biomedical Science Tags: Immunology and Inflammation Source Type: research
Title: What Happens if You Go Untreated for Lyme Disease?Category: Diseases and ConditionsCreated: 6/17/2020 12:00:00 AMLast Editorial Review: 6/29/2020 12:00:00 AM
Source: MedicineNet Arthritis General - Category: Rheumatology Source Type: news
LOW SEROPREVALENCE OF LYME DISEASE AMONG MULTIPLE SCLEROSIS PATIENTS IN NEW BRUNSWICK. Can J Neurol Sci. 2020 Jun 29;:1-11 Authors: MacLean G, Cook P, Lindsay LR, Hatchette TF, Webster D PMID: 32594964 [PubMed - as supplied by publisher]
Source: The Canadian Journal of Neurological Sciences - Category: Neurology Authors: Tags: Can J Neurol Sci Source Type: research
Authors: Franco-Avecilla D, Yeung C, Baranchuk A PMID: 32575061 [PubMed - in process]
Source: Canadian Medical Association Journal - Category: General Medicine Tags: CMAJ Source Type: research
Authors: Semproni M, Rusk R, Wuerz T PMID: 32575059 [PubMed - in process]
Source: Canadian Medical Association Journal - Category: General Medicine Tags: CMAJ Source Type: research
LYME disease is a bacterial infection that can be spread to humans by infected ticks, tiny spider-like creatures that dwell in woodland. Untreated Lyme disease can cause a host of debilitating health problems so it is vital to act on the early warning sign.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Compared to the standard two-tiered testing (STTT) algorithm for Lyme disease serology using an enzyme immunoassay (EIA) followed by Western blotting, data from the United States suggest that a modified two-tiered testing (MTTT) algorithm employing two EIAs has improved sensitivity to detect early localized Borrelia burgdorferi infections without compromising specificity. From 2011 to 2014, in the Canadian province of Nova Scotia, where Lyme disease is hyperendemic, sera submitted for Lyme disease testing were subjected to a whole-cell EIA, followed by C6 EIA and subsequently IgM and/or IgG immunoblots on sera with EIA-pos...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
Discussion Bradycardia is a heart rate below what the lowest value that is normal for age. Infants and children have higher heart rates that slowly decrease with age to adult levels. It is usually noted as an incidental finding because of increased vagal tone. Reasons for cardiology referral include associated heart murmur, syncope especially if associated with exercise or unusual triggers, other signs such as chest pain or palpitations, family history of sudden cardiac death, congenital heart disease or familial heart disease, bradycardic medication use, or unusual symptoms associated with the concern. Severe bradycardia ...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
More News: Chemistry | Laboratory Medicine | Lyme Disease | Microbiology | Study