Preparation of self-assembled nanoparticles of ɛ-polylysine - sodium alginate: a sustained-release carrier for antigen delivery

Publication date: Available online 25 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jing Yuan, Lu Guo, Sijia Wang, Dan Liu, Xia Qin, Lili Zheng, Chunlian Tian, Xiaohu Han, Ran Chen, Ronghuan YinAbstractLow immunogenicity prohibits the widespread use of subunit vaccine against infectious diseases and cancers. Hence, a new generation of adjuvants and delivery systems is indispensable for more potent antigen-specific immune responses. Predominantly, nanoparticles formulated from biodegradable polymers are being widely explored as carriers of novel vaccines owing to their outstanding natural properties. We fabricated a model antigen - bovine serum albumin (BSA) encapsulated ε-polylysine (ε-PL) - sodium alginate (SA) nanoparticles (PSNPs), which were self-assembled by ionotropic complexation method, a very simple and mild process, as a result of the electrostatic interaction between oppositely charged polyelectrolyte complexes (PEC). After the preparation, various in vitro parameters were characterized. Scanning electron microscope and dynamic light scattering were employed to study the morphology, size, zeta potential and optimize formulation. Forming mechanism of PSNPS was analyzed and verified by infrared absorption spectra and thermal analysis. Delivery behavior of PSNPs was assessed via release study, cytotoxicity measurement and cellular uptake. BSA-PSNPs with a mean particle diameter 133.2 ± 0.5 nm, narrow size distribution and negatively charge...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research