Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants.

Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants.
Curr Protoc Hum Genet. 2018 Apr;97(1):e58
Authors: Tai AC, Parfenov M, Gorham JM
Abstract
DNA structural variants can be analyzed by droplet digital PCR (ddPCR), a water-oil microfluidics and fluorescence technology to quantify target nucleic acids with extreme precision and sensitivity. Traditional ddPCR uses expensive fluorescent oligonucleotide probes that require extensive optimization. Here we describe a variation of ddPCR using a DNA-binding dye (EvaGreen), whose properties allow target products to be effectively quantified at a significantly lower cost. © 2018 by John Wiley & Sons, Inc.
PMID: 30040209 [PubMed - in process]

Source: Current Protocols in Human Genetics - July 25, 2018 Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research