[Determination of Histamine by a Rapid Fluorescamine-UPLC Method].

[Determination of Histamine by a Rapid Fluorescamine-UPLC Method]. Shokuhin Eiseigaku Zasshi. 2018;59(3):121-125 Authors: Murakami Y, Nakai N, Ando N, Okayama A Abstract We have developed a rapid analysis method for determination of histamine using ultra-performance liquid chromatography and fluorescamine derivatization. Histamine was extracted from foods with trichloroacetic acid after homogenization. The supernatant obtained by centrifugation was filtered and derivatized without solid-phase extraction. The mobile phase was 10 mM phosphate-acetonitrile (75 : 25) without ion-pairing. Recovery tests of histamine from fishes, seasoning and fish products gave acceptable recovery (95.8-117.7%). This method should be useful for rapid evaluation of food poisoning outbreaks. PMID: 30033988 [PubMed - in process]
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research