Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives

Publication date: Available online 25 July 2018Source: Stem Cell ResearchAuthor(s): T.H. Kasai-Brunswick, D. Silva dos Santos, R.P. Ferreira, D.S. Araujo, G.M. Dias, J.L.A. Coutinho, F.E.S.F. Cruz, E.B. Sternick, F. Gubert, J.C.G. Oliveira, I.M. Vaz, T. Borgonovo, P.R.S. Brofman, R.S. Moura-Neto, R. Silva, A.C. Campos-de-Carvalho, A.B. CarvalhoAbstractFour human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity.

Source: Stem Cell Research - July 25, 2018 Category: Stem Cells Source Type: research