Highly selective and sensitive detection of Staphylococcus aureus with gold nanoparticle-based core-shell nano biosensor

Publication date: Available online 25 July 2018Source: Molecular and Cellular ProbesAuthor(s): Reza Shahbazi, Mojtaba Salouti, Bahram Amini, Ahmad Jalilvand, Ebrahim Naderlou, Ali Amini, Arash ShamsAbstractStaphylococcus aureus is a gram-positive and opportunistic pathogen that is one of the most common causes of nosocomial infections; therefore, its rapid diagnosis is important and valuable. Today, the use of nanoparticles is expanding due to their unique properties. The purpose of the present study is the determination of S. aureus by a colorimetric method based on gold nanoparticles (AuNPs). Firstly, S. aureus was cultured on both LB media (broth and agar) and their chromosomal DNA was extracted. Afterwards, primers and biosensors were designed based on Protein A sequence data in the gene bank. PCR assay was performed under optimal conditions and the PCR product was electrophoresed on 2-percent agarose gel. The synthesized biosensors were conjugated with AuNPs and, eventually, a single-stranded genome was added to the conjugated AuNPs and hybridization was performed. The results were evaluated based on color change detected by the eye, optical spectrophotometry, and transient electron microscopy. Finally, the sensitivity and specificity of the biosensor were determined. The results of the present study showed a 390 bp band on the agarose electrophoresis gel, which confirmed the presence of Protein A genes on the chromosome of the bacteria. The PCR and colorimetric methods ...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research