Combination of phase-solubility method and capillary zone electrophoresis to determine binding constants of cyclodextrins with practically water-insoluble compounds

Publication date: Available online 21 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Viktoria V. Sursyakova, Nikolai G. Maksimov, Vladimir A. Levdansky, Anatoly I. RubayloAbstractThe combined method based on phase-solubility technique and capillary zone electrophoresis (PS-CZE) was suggested for the determination of binding (stability) constants of cyclodextrins (CD) complexes with water-insoluble organic compounds that have no or weak UV chromophores. In this method, the insoluble compounds are agitated at the desired temperature in CD solutions with different concentration up to the attainment of equilibrium and then CZE is used to determine the concentration of the compounds that have passed into the solutions. To avoid precipitation and complex dissociation, the background electrolyte (BGE) for CZE should contain ethanol and, if necessary, cyclodextrin. The samples should be diluted with the BGE without CD so that the CD concentrations in BGE and samples were equal to preclude a baseline shift. Using the suggested approach, the inclusion complexes between betulinic and betulonic acids, pentacyclic lupane-type triterpenes, and hydroxypropyl-β- and γ-cyclodextrins (HP-β-CD and HP-γ-CD) were studied. It was found that solubility of the acids studied in HP-β-CD solutions did not differ from their solubility in pure water. That is, the HP-β-CD complexes of the acids studied were not formed in noticeable amount. At t...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 20 January 2020Source: Molecular and Cellular EndocrinologyAuthor(s): Ronald J. van der Sluis, Menno HoekstraAbstractAdrenal-derived glucocorticoids mediate the physiological response to stress. Chronic disturbances in glucocorticoid homeostasis, i.e. in Addison's and Cushing's disease patients, predispose to the development of atherosclerotic cardiovascular disease. Here we review preclinical and clinical findings regarding the relation between changes in plasma glucocorticoid levels and the atherosclerosis extent. It appears that, although the altered glucocorticoid function can in most...
Source: Molecular and Cellular Endocrinology - Category: Endocrinology Source Type: research
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Source: Gynecological Endocrinology - Category: Endocrinology Authors: Source Type: research
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Source: Gynecological Endocrinology - Category: Endocrinology Authors: Source Type: research
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Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research
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Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
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Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
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Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
Source: Core Evidence - Category: Drugs & Pharmacology Tags: Core Evidence Source Type: research
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
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Source: Acta Pharmaceutica Sinica B - Category: Cancer & Oncology Source Type: research
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