Cross-platform metabolic profiling deciphering the potential targets of Shenfu injection against acute viral myocarditis in mice

Publication date: 25 October 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 160Author(s): Guangguo Tan, Qian Zhou, Kui Liu, Xin Dong, Ling Li, Wenting Liao, Hong WuAbstractAcute viral myocarditis (AVMC) is typically caused by cardiotropic viral infection. There is a paucity of specific treatment options available with proven efficacy. Chinese patented pharmaceutical product Shenfu injection (SFI) has potent efficacy on treating AVMC in clinical practice. However, the molecular mechanism is still unknown. We employed cross-platform metabolomics combined with computational systems analysis, based on reversed-phase liquid chromatography–mass spectrometry (RPLC-MS), hydrophilic interaction liquid chromatography–mass spectrometry (HILIC-MS) and gas chromatography-mass spectrometry (GC–MS), to deciphering the targeted metabolic pathways of SFI against AVMC induced by coxsackievirus B3 (CVB3). Quantitative real-time PCR (qRT-PCR) technique was further applied to determining the expressions of the key genes associated with the SFI-targeted metabolic pathways. We have identified 48 significantly changed metabolites related to CVB3-induced AVMC, and SFI can significantly regulate the abnormalities of 33 metabolites and 9 relevant enzymes. Combined metabolic pathway enrichment and topology analyses revealed that the mechanisms of SFI against CVB3-induced AVMC may be attributed to modulating the disordered homeostasis of sphingolipid metabolism, g...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 10 November 2019Source: Revista Clínica Española (English Edition)Author(s): G. Serralta San Martín, J. Canora Lebrato, en representación del Grupo de Trabajode Ecografía Clínica y del Grupo de Trabajo de Enfermedades Autoinmunes Sistémicas de la Sociedad Españ ola de Medicina InternaAbstractClinical ultrasonography should be considered a complementary examination that can change clinical practice, as well as a tool to add to the medical history.Systemic autoimmune diseases (SAD) can involve numerous structures and organs. Ultrasonog...
Source: Revista Clinica Espanola - Category: Internal Medicine Source Type: research
Acute myocarditis is an inflammatory disease of the myocardium most often resulting from a viral infection or autoimmune disorders.1,2 Among the other potential causes myocarditis can be due to hypersensitivity reactions,3 or to the inhibition of immune checkpoints.4,5 Post mortem examinations in young adults have demonstrated that acute myocarditis was responsible of 3-12% of cases of sudden cardiac death.6,7 As most previous studies of acute myocarditis comprised a relatively small patient population (100 to 200) with variable timing of diagnosis from symptom onset,8,9 the in-hospital as well as long term outcomes of pat...
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Clinical Research Study Source Type: research
Immune-checkpoint inhibitors (ICIs) are novel therapeutic agents that have demonstrated relevant anti-tumor responses in multiple cancer types. They produce a potentiation of the immune response against the cancer by blocking the negative down-regulation of T cells. Checkpoint inhibitor-associated neurological side effects are rare but also potentially life-threatening [1].
Source: Neuromuscular Disorders - Category: Neurology Authors: Tags: Case report Source Type: research
Abstract Huge variations exist in cardiology training programs across the world. In developing (middle-income) countries, such as Brazil, to find the right balance between training improvements and social and economic conditions of the country may be a difficult task. Adding more training years or different mandatory rotations, for instance, may be costly and not have an immediate direct impact on enhancing patient care or public health. In this text, we compare the Brazilian cardiology training system with other proposals implemented in developed countries from North America and Europe, aiming to point out issues worth of...
Source: Arquivos Brasileiros de Cardiologia - Category: Cardiology Source Type: research
Cardiogenic shock continues to have unacceptably high in-hospital mortality of over 50% and ST-segment elevation myocardial infarction (STEMI) is the leading cause of cardiogenic shock among other causes such as myocarditis, non-ST-elevation myocardial infarction, acute exacerbation of chronic heart failure, and pulmonary embolism.1 STEMI resulting in left ventricular failure accounts for 70 –80% of STEMI complicated with cardiogenic shock (STEMI-CS) and the prevalence of STEMI-CS was 6.5 to 10.1% in the United States between 2003–2010.
Source: Heart and Lung - Category: Intensive Care Authors: Source Type: research
Authors: Wang CR, Tsai YS, Tsai HW Abstract The presence of cardiac insufficiency is recognized as a poor prognostic factor in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV)1, and heart failure is an identified mortality risk factor for eosinophilic granulomatosis with polyangiitis (EGPA)2. PMID: 31676698 [PubMed - as supplied by publisher]
Source: Journal of Rheumatology - Category: Rheumatology Tags: J Rheumatol Source Type: research
AbstractCardiac involvement is a major cause of morbidity and mortality in TA (2 ‐4). Any structure of the heart can potentially be affected with predominant involvement of ascending aorta leading to aortic aneurysm with aortic regurgitation, coronary vasculitis and myocarditis leading to dilated cardiomyopathy. Nearly half of the patients have cardiac involvement at some poin t during the course of their disease (5‐7).
Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Tags: Reply To Letter To The Editor Source Type: research
We report an uncommon case of myocarditis complicated with permanent CAVB caused by Escherichia coli (E coli) bacteremia. Patient concerns: A 77-year-old woman presented at the emergency department with chest pain, dizziness, nausea, and cold sweats of 1-day duration. She had histories of type 2 diabetes mellitus, hyperlipidemia, and chronic kidney disease with regular medical therapy. Diagnosis: Both blood and urine cultures were positive for E coli. Regional inferior wall motion abnormalities on echocardiography, unexplained life-threatening arrhythmias, newly abnormal electrocardiogram, elevated cardiac troponins,...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
as IS Abstract We investigated the immunomodulatory, antiparasitic and cardioprotective effects of a sesquiterpene lactone (SL) administered alone or combined with benznidazole (Bz), in a murine model of Chagas' disease by in vitro and in vivo assays. Antiparasitic and cytotoxic potential of tagitinin C (SL) and Bz were tested in vitro against T. cruzi epimastigotes and cardiomyocytes. Swiss mice challenged with T. cruzi were also treated for 20 days with tagitinin C (10 mg/kg) alone and combined with Bz (100 mg/kg). Tagitinin C exhibited a higher antiparasitic (IC50: 1.15 µM) and cytotoxic (CC50 at ...
Source: International Immunopharmacology - Category: Allergy & Immunology Authors: Tags: Int Immunopharmacol Source Type: research
In this study, TLR3 and TRAF6 were identified as the targets of miR-146a. The elevated miR-146a inhibited NF-κB translocation and subsequently down-regulated proinflammatory cytokine expression in the CVB3-infected cells. Therefore, the NF-κB pathway can be doubly blocked by miR-146a through targeting of TLR3 and TRAF6. MiR-146a may be a negative regulator on inflammatory response and an intrinsic protective factor in CVB infection. PMID: 31676570 [PubMed - as supplied by publisher]
Source: RNA - Category: Genetics & Stem Cells Authors: Tags: RNA Source Type: research
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