Nandrolone alter left ventricular contractility and promotes remodelling involving calcium-handling proteins and renin-angiotensin system in male SHR

Publication date: Available online 21 July 2018Source: Life SciencesAuthor(s): Antonio F. Melo Junior, Polyana L.M. Dalpiaz, Glauciene J. Sousa, Phablo Wendell C. Oliveira, Antônio M. Birocale, Tadeu U. Andrade, Gláucia R. Abreu, Nazaré S. BissoliAbstractAimsHypertension is a highly prevalent disease that has been correlated to severe organ damage and mortality. However, the role of androgens in hypertension is controversial. The aim of this study was to evaluate the cardiac effects of the nandrolone decanoate (NDL) in male SHR.Main methodsAt 12 weeks of age, male SHR rats were separated into three groups: Control (CON), Nandrolone 10 mg/kg twice weekly (NDL), and NDL plus Enalapril 10 mg/kg/day (NDL-E) groups. The animals were treated for 4 weeks. Haemodynamic parameters were acquired through ventricular catheter implantation. The left ventricle was stained with haematoxylin/eosin or picrosirius red. Western blot analysis of TNF-α, ACE, AT1R, β1-AR, PLB, p-PLBser16 and SERCA2a was performed.Key findingsNandrolone increased hypertension in SHR rats and enalapril reduced blood pressure to values below those of the control. NDL increased +dP/dtmax, −dP/dtmax and cardiac hypertrophy, which were prevented in the NDL-E group. Cardiac collagen deposition was increased in the NDL group, with this effect being attenuated by enalapril in NDL-E animals. TNF-α, ACE, AT1R and β1-AR proteins were increased in the NDL, and enalapril decreased them, except for TNF-α. The r...
Source: Life Sciences - Category: Biology Source Type: research