Comparative extraction and simple isolation improvement techniques of active constituents’ momilactone A and B from rice husks of Oryza sativa by HPLC analysis and column chromatography

Publication date: Available online 20 July 2018Source: Saudi Pharmaceutical JournalAuthor(s): Ateeque Ahmad, Tran Dang Xuan, Truong Ngoc Minh, Nasir Ali Siddiqui, Nguyen Van QuanAbstractThis paper reports comparative extraction efficiencies and enhancement methods for natural herbicidal (growth inhibitors) compounds, momilactone A and B, respectively from the dried husks of Oryza sativa using different extraction techniques and different solvent systems. Four different extraction techniques viz. percolation, agitation with heat, sonication and soxhlet using five solvent systems as ethyl acetate, acetone, acetonitrile, methanol and methanol:water (8:2) were evaluated. In these studies, it was observed that maximum extract yield was obtained using in methanol and methanol/water mixture as extracting solvent by soxhlet technique although the content of total momilactones A and B was higher in the methanol/water mixture in comparison to other extractions. The successive and simple isolation enrichment technique for momilactones A and B were achieved by solid-matrix partitioning after the treatment of methanolic extract with charcoal and using ethyl acetate as extracting solvent for momilactones A and B. The quantitative analysis of the extraction and enrichment development protocol was validated by a simple, accurate, reproducible RP-HPLC-UV-VIS method using a binary gradient elution comprising of acetonitrile and water (70:30). The separation was achieved on a waters Spherisorb ...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research