A sensitive fluorescence “turn-off-on” biosensor for poly(ADP-ribose) polymerase-1 detection based on cationic conjugated polymer-MnO2 nanosheets

Publication date: 10 November 2018Source: Sensors and Actuators B: Chemical, Volume 273Author(s): Shuangshuang Wu, Changhui Chen, Haitang Yang, Wei Wei, Min Wei, Yuanjian Zhang, Songqin LiuAbstractPoly(ADP-ribose) polymerase-1 (PARP-1) monitoring has attracted extensive attention because it serves a vital role in human pathologies. However, only a few researches about its detection methods have been reported because PARP-1 and its catalyzed product poly(ADP-ribose) polymer (PAR) are lack of optical or electrochemical activity. Herein, a convenient fluorescence “turn-off-on” nanosensor based on cationic conjugated polymer (PFP) and MnO2 nanosheets has been designed for selective detection of PARP-1 in vitro. To the best of our knowledge, it is the first time to use manganese dioxide (MnO2) nanosheets for PARP-1 detection. The fluorescence intensity of PFP can be quenched by MnO2 nanosheets via a fluorescence resonance energy transfer (FRET). While the subsequently joined electronegative poly(ADP-ribose) polymer (PAR), catalysate of PARP-1, take positively charged MnO2 nanosheets away from PFP via electrostatic interaction, causing sufficient recovery of fluorescent signal. The sensing platform displayed a sensitive response to PARP-1 in a linear range of 0.03 − 1.5 U (0.024 − 1.2 nM), with a detection limit of 0.004 U (0.003 nM), which is 10–100 lower than reported methods. As expected, this method was successfully applied to the detection of PARP-1 in hu...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research