Cells of ng2 lineage increase in glomeruli of mice following podocyte depletion.

CELLS OF NG2 LINEAGE INCREASE IN GLOMERULI OF MICE FOLLOWING PODOCYTE DEPLETION. Am J Physiol Renal Physiol. 2018 Jul 18;: Authors: Suzuki T, Eng DG, McClelland AD, Pippin JW, Shankland SJ Abstract Under certain circumstances, podocytes can be partially replaced following their loss in disease. The inability of podocytes to proliferate suggests that replacement derives from other cell types. Because Neural/glial antigen 2 (NG2) expressing cells can serve as progenitors in other organs, and because herein we showed increased NG2 staining in podocytes following their loss in experimental FSGS, we used lineage tracing in NG2-CreER tdTomato mice to test the hypothesis that partial podocyte replacement might derive from this cell population. The percentage of glomeruli with RFP-labeled NG2 cells increased following podocyte depletion, which was augmented by enalapril. However, BrdU was not detected in RFP-labeled cells, consistent with the migration of these cells to the glomerulus. Within glomeruli, RFP-labeled cells did not co-express podocyte proteins (p57, synaptopodin, nephrin or podocin), but did co-express markers for mesangial (α8 Integrin, PDGFβ Receptor) and parietal epithelial cells (PAX8, SSeCKS). These results suggest that following podocyte depletion, cells of NG2 lineage do not serve as adult podocyte progenitors, but have the ability to transdifferentiate to mesangial and parietal epithelial cell fates. PMID: 300...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research