Combination of tenofovir and emtricitabine with efavirenz does not moderate inhibitory effect of efavirenz on mitochondrial function and cholesterol biosynthesis in human T lymphoblastoid cell line.

Combination of tenofovir and emtricitabine with efavirenz does not moderate inhibitory effect of efavirenz on mitochondrial function and cholesterol biosynthesis in human T lymphoblastoid cell line.
Antimicrob Agents Chemother. 2018 Jul 16;:
Authors: Li M, Sopeyin A, Paintsil E
Abstract
Efavirenz (EFV), the most popular non-nucleoside reverse transcriptase inhibitor, has been associated with mitochondrial dysfunction in most in vitro studies. However, in real life the prevalence of EFV-induced mitochondrial toxicity is relatively low. We hypothesized that the agents given in combination with EFV might moderate the effect of EFV on mitochondrial function. To test this hypothesis, we cultured human T lymphoblastoid cell line (CEM cells) with EFV alone and in combination with emtricitabine (FTC) and tenofovir disoproxil fumarate (TDF) to investigate the effects on mitochondrial respiration and function and cholesterol biosynthesis. There was a statistically significant concentration- and time-dependent apoptosis, reduction in mitochondrial membrane potential (ΔΨ), and increase production of reactive oxygen species (ROS) in cells treated with either EVF alone or in combination with TDF/FTC. EFV treated cells compared to DMSO treated cells had significant reduction in oxygen consumption rate (OCR) contributed by basal mitochondrial respiration and decreased protein expression of electron transport chain complexes (CI, CII, and CIV). Tre...

Source: Antimicrobial Agents and Chemotherapy - July 16, 2018 Category: Microbiology Authors: Li M, Sopeyin A, Paintsil E Tags: Antimicrob Agents Chemother Source Type: research