Ulipristal acetate induces cell cycle delay and remodeling of extracellular matrix.

Ulipristal acetate induces cell cycle delay and remodeling of extracellular matrix.
Int J Mol Med. 2018 Jul 17;:
Authors: Shin SJ, Kim J, Lee S, Baek J, Lee JE, Cho C, Ha E
Abstract
Uterine leiomyoma is a benign tumor that grows within the muscle tissue of the uterus. Ulipristal acetate (UPA) is a pre‑operative drug used to reduce the size of leiomyoma. The aim of the present study was to examine the in vitro mechanistic details of action of UPA on uterine leiomyomas. Primary cultures of leiomyoma cells were isolated from patient myomectomy specimens and incubated in the presence or absence of UPA at various concentrations. The proliferation, cell viability and doubling time properties of the treated cells were analyzed. In addition, the mRNA and protein expression levels of p21, p27, cyclin E, cyclin‑dependent kinase 2 (CDK2), matrix metalloproteinase (MMP)‑2 and MMP‑9 were examined, as well as the structure of F‑actin in the primary‑cultured leiomyoma cells. The results demonstrated that UPA exerted inhibitory effects on proliferation of primary‑cultured leiomyoma cells. Expression of p21 and p27 was upregulated, while cyclin E and CDK2 were downregulated in UPA‑treated primary‑cultured leiomyoma cells. An increased expression of MMP‑2 was observed in primary‑cultured leiomyoma cells and a leiomyoma tissue sample of a patient with previous history of UPA treatment. Furthermore, a pronounced formation of Fâ...

Source: International Journal of Molecular Medicine - July 17, 2018 Category: Molecular Biology Authors: Shin SJ, Kim J, Lee S, Baek J, Lee JE, Cho C, Ha E Tags: Int J Mol Med Source Type: research