Development of a disposable electrochemical sensor for detection of cholesterol using differential pulse voltammetry

In this study, a sensitive and selective electrochemical sensor was fabricated by using a screen printed carbon electrode (SPCE), multi-walled carbon nanotubes (MWCNTs) and β-cyclodextrin (β-CD) for detecting cholesterol. MWCNTs were functionalized with benzoic acid moiety by employing diazonium salt chemistry, and, subsequently, a thin film of functionalized CNTs were coated on the surface of SPCE. Afterwards, β-CD was immobilized on functionalized MWCNTs modified SPCE which acts as a host to recognize guest (cholesterol) molecule specifically. Under the optimal experimental conditions and using differential pulse voltammetry (DPV) as transduction technique the sensor was able to detect cholesterol level ranges from 1 nM to 3 μM, with a detection limit of 0.5 nM. Specificity of the developed sensor towards target analyte (cholesterol) was confirmed in the presence of common interfering species including glucose, uric acid and ascorbic acid. The applicability of proposed sensor was also demonstrated for cholesterol determination in human serum samples with good recovery results (94-96%) and maximum RSD (relative standard deviation) of 4.5%.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Conclusions: Our findings suggest that some subsets of XPD patients may be at risk of radiosensitivity reactions and treatment with statins and bisphosphonates may be an interesting approach of radioprotection countermeasure. Different mechanistic models were discussed to better understand the potential specificity of the p.[Arg683Trp];[Arg616Pro] XPD mutations . PMID: 31738647 [PubMed - as supplied by publisher]
Source: International Journal of Radiation Biology - Category: Radiology Tags: Int J Radiat Biol Source Type: research
Conclusions Patients are not adequately ready to change their health-related behaviors. Higher cardiovascular risk predicts higher readiness to change health behaviors.
Source: Journal of Nursing Research - Category: Nursing Tags: ORIGINAL ARTICLES Source Type: research
Publication date: Available online 19 November 2019Source: Journal of EthnopharmacologyAuthor(s): Lujie Fan, Ying Peng, Dan Wu, Jianhong Hu, Xin'e Shi, Gongshe Yang, Xiao LiAbstractEthnopharmacological relevanceMulberry leaves are the dry leaves of Morus nigra L. trees, which are widely cultivated in central and southern China. Mulberry has a long history of medicinal use, such as anti-stress, lowering blood glucose and anti-obesity.Aim of the studyExplore the effects of mulberry leaves on fat deposition as well as the underlying mechanisms.Materials and methodsTotal of 48 fattening pigs weighing about 70 kg were randoml...
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 19 November 2019Source: Journal of Ayurveda and Integrative MedicineAuthor(s): Rekha Khandia, Chandra Shekhar Pathe, Pratibha Vishwakarma, Kuldeep Dhama, Ashok MunjalAbstractBackgroundInsecticides are widely used in agriculture to curb the loss caused by insects. These insecticides are incorporated into the food chain and accumulate in the human body, as well disturb the various metabolic pathways. Imidacloprid is an insect neurotoxin commonly used in agriculture to control the insect pests. P. niruri is a traditional medicinal shrub widely used as an anti-inflammatory, antipyretic, and a...
Source: Journal of Ayurveda and Integrative Medicine - Category: Complementary Medicine Source Type: research
ConclusionsThe study has provided a way out to combat the toxicity effects associated with bulk statins by synthesis of nanoparticles of statins with more efficacy, more bioavailability, more solubility and more radical scavenging capacity even in lesser dose.Graphical abstract
Source: European Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Authors: El Menshawe SF, Nafady MM, Aboud HM, Kharshoum RM, Elkelawy AMMH, Hamad DS Abstract The current study aimed to encapsulate fluvastatin sodium (FVS), a member of the statins family possessing pleiotropic effects in rheumatoid arthritis (RA), into spanlastic nanovesicles (SNVs) for transdermal delivery. This novel delivery could surmount FVS associated oral encumbrances such as apparent first-pass effect, poor bioavailability and short elimination half-life, hence, accomplishing platform for management of RA. To consummate this objective, FVS-loaded SNVs were elaborated by thin film hydration method, utilizi...
Source: Drug Delivery - Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
Authors: Florentin M, Kostapanos MS, Anagnostis P, Liamis G Abstract Introduction: Hypertriglyceridemia is associated with both the development of cardiovascular disease (CVD) when mild-to-moderate and high risk of pancreatitis when more severe. The residual CVD risk after low-density lipoprotein cholesterol (LDL-C) lowering is, in part, attributed to high triglyceride (TG) levels. Therefore, there appears to be a need for effective TG-lowering agents.Areas covered: This review presents the most recent advances in hypertriglyceridemia treatment; specifically, it discusses the results of clinical trials and critical...
Source: Expert Opinion on Pharmacotherapy - Category: Drugs & Pharmacology Tags: Expert Opin Pharmacother Source Type: research
Authors: Babinska A, Kaszubowski M, Kmieć P, Sworczak K Abstract BACKGROUND: Adipose tissue secretes many adipokines and cytokines, which may be an additional risk factor of cardiovascular and metabolic diseases in patients with an incidentally discovered pheochromocytoma (PHEO). The aim of the study was to investigate levels of selected adipocytokines in these patients. METHODS: This prospective study included 12 patients with an incidentally discovered PHEO and 18 healthy participants. In all participants plasma/serum concentrations of triglycerides, HDL and LDL cholesterol, insulin, glucose, adipocytokines ...
Source: Minerva Endocrinologica - Category: Endocrinology Tags: Minerva Endocrinol Source Type: research
AbstractPurpose of ReviewTo systematically examine the association between low-density lipoprotein cholesterol (LDL-C) and risk of hemorrhagic stroke.Recent FindingsA previous meta-analysis of prospective studies published in 2013 showed that higher concentrations of LDL-C were associated with lower risk of hemorrhagic stroke. Recently, seven large cohort studies were published examining LDL-C and risk of hemorrhagic stroke in different populations.SummaryTwelve prospective studies with 476,173 participants and 7587 hemorrhagic stroke cases were included in the current meta-analysis. The results showed that a 10 mg/dL incr...
Source: Current Atherosclerosis Reports - Category: Cardiology Source Type: research
Background: The development of strategies that could not only efficiently detect the onset of Amyotrophic Lateral Sclerosis (ALS), a fatal neurodegenerative disorder with no cure but also predict its development and evaluate therapeutic intervention would be of great value. In this respect, the metabolic status of ALS patients has called attention. Hence, this study aimed to investigate the potential correlation between changes in ALS's metabolic parameters with the disease outcome in a systematic review.Methods: The manuscripts were manually searched within different databases (PubMed, Web of Science and Cochrane). The in...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
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