ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO-).

ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO-).
Chem Commun (Camb). 2018 Jul 16;:
Authors: Wu L, Yang Q, Liu L, Sedgwick AC, Cresswell AJ, Bull SD, Huang C, James TD
Abstract
ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO- through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO- (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO- in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg2+ and H2O2. Interestingly, Hg2+ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO- in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO- in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO- in drinking water samples.
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Source: Chemical Communications - July 16, 2018 Category: Chemistry Authors: Wu L, Yang Q, Liu L, Sedgwick AC, Cresswell AJ, Bull SD, Huang C, James TD Tags: Chem Commun (Camb) Source Type: research