Development of a thrombin generation test in cultured endothelial cells: Evaluation of the prothrombotic effects of antiphospholipid antibodies

Circulating antiphospholipid antibodies (APL) induce vascular injury and endothelial dysfunction, which are associated with thrombotic events and/or fetal loss. We developed a model in which calibrated automated thrombin generation (CAT) is carried out in wells lined with cultured endothelial cells. Then we investigated how far b2GP1 antibodies provoked thrombin generation (TG) enhancing effects in these cells and/or in blood platelets.

Source: Thrombosis Research - July 16, 2018 Category: Hematology Authors: Paul Billoir, S ébastien Miranda, Louise Damian, Vincent Richard, Ygal Benhamou, Véronique Le Cam Duchez Tags: Full Length Article Source Type: research