Marking of definitive HSC precursors in E7.5 –E8.5 embryos using an Abcg2-CreER lineage-tracing mouse model

Abcg2 is a plasma membrane transporter that is expressed in the side population cells of a variety of tissues, including cancer cells, and is required for their SP phenotype [1,2]. In adult mice, virtually all hematopoietic stem cells (HSCs) express Abcg2 [3]. Lineage-tracing studies using an Abcg2CreERRosaYFP allele in the adult mice confirmed expression of Abcg2 in adult HSCs and revealed that adult tissue-specific intestinal stem cells and spermatogonial stem cells also express Abcg2 [4]. Definitive HSCs (dHSCs) have been identified at embryonic day 10.5 (E10.5) in the midgestation dorsal aorta with an estimated total of
Source: Experimental Hematology - Category: Hematology Authors: Tags: Brief Communication Source Type: research