GSE114463 Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach

In this study, the model strain C. difficile 630 Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells for the duration tested. However, an extensive change in gene expression was determined by RNA-Seq with 49 effected genes after 15 min and more than 400 after one hour. Effected gene s exhibit a wide range of functionality, including the metabolism of amino acids, enzymes for cell wall synthesis, the uptake and metabolism of sugars, synthesis of B vitamins as well as purine synthesis and DNA repair. Our study suggests that tolerance of C. difficile towards O2 is based on a compl ex and far-reaching adjustment of global gene expression.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Clostridioides difficile Source Type: research