Epitope Mapping of Anti-Telomerase Reverse Transcriptase Monoclonal Antibodies.

Epitope Mapping of Anti-Telomerase Reverse Transcriptase Monoclonal Antibodies. Monoclon Antib Immunodiagn Immunother. 2018 Jul 13;: Authors: Furusawa Y, Itai S, Yamada S, Kaneko MK, Kato Y Abstract Telomerase reverse transcriptase (TERT) is the main catalytic subunit of telomerase-associated protein machinery. Upregulation of TERT at the transcriptional level results in immortal cell phenotype associated with cancer. To date we have developed eight anti-TERT monoclonal antibodies (mAbs) (TMab-4, TMab-5, TMab-6, TMab-7, TMab-9, TMab-10, TMab-11, and TMab-12) by immunizing mice with synthetic peptides (302-QHHAGPPSTSRPPRPWDTPC-321) of TERT. We further characterized those epitopes using enzyme-linked immunosorbent assay, and here we discuss the critical epitope of an anti-TERT mAb, which is applicable for immunohistochemical analysis. PMID: 30004263 [PubMed - as supplied by publisher]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research