Surface-dependent endocytosis of poly(isobutylcyanoacrylate) nanoparticles by Trichomonas vaginalis

Publication date: 5 September 2018Source: International Journal of Pharmaceutics, Volume 548, Issue 1Author(s): Sophia Malli, Christian Bories, Mickaël Bourge, Philippe.M. Loiseau, Kawthar BouchemalAbstractPrevious data from our research group showed that chitosan-coated poly(isobutylcyanoacrylate) nanoparticles (NPs) (denoted PIBCA/Chito20) exhibited intrinsic anti-Trichomonas vaginalis activity, while PIBCA/pluronic® F68 without chitosan (PIBCA/F68) were inactive. However, the mechanism of anti-T. vaginalis activity of chitosan-coated PIBCA NPs is still unknown. Our hypothesis is that chitosan-coated NPs are internalized by the parasite, contrarily to PIBCA/F68. In this investigation, the impact of NP surface on their internalization by the protozoan was studied using flow cytometry and parasite morphological changes after different incubation times with PIBCA/Chito20 NPs were monitored by electron microscopy. Flow-cytometry revealed that PIBCA/Chito20 NPs were uptaken by T. vaginalis as early as 10-min-incubation. Drastic cell morphological transformations were observed from scanning electron microscopy and transmission electron microscopy after incubation with PIBCA/Chito20 NPs. Numerous pits were seen on cell membrane since 10 min. Gradual increase in contact time increased NP endocytosis and induced proportional damages to T. vaginalis membrane. Then, investigation of whether PIBCA/Chito20 NPs can improve MTZ anti-T. vaginalis activity was studied using check...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Conclusions Nucleoside hydrolases are vital enzymes for the replication of Leishmania, conserved phylogenetic marker of the genus and strong-specific immunogens. We demonstrated that NH36 is an excellent target for chemotherapy of visceral leishmaniasis. Searching for the most immunogenic fraction of promastigotes of Leishmania we described the FML antigen of L. (L.) donovani, that has as its main component, the NH36 Nucleoside hydrolase. We developed second–generation vaccines with the FML and the NH36 native antigens, and with the NH36 recombinant protein. In addition, we obtained a third generation vaccine based ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In conclusion, we hope that this review brings about a new and more in-depth understanding of the part that Leishmania exosomes and various infectious agents play in the context of host-parasite interactions, with a particular focus on the establishment of infection. Future research in this field of investigations is critical for the development of new vaccine and diagnostic tools. Author Contributions The first draft was done by MO. AF and GD added new information. Funding Research in MO laboratory is supported by the Canadian Institute of Health Research (grant number: PJT-159765) and the Natural Sciences and Enginee...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Publication date: Available online 7 April 2019Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Kathryn Purple, Todd Amacker, Chauntelle Williams, Richard GerholdAbstractWater containing organic material has been shown to increase the persistence of the avian pathogenic protozoa, Trichomonas gallinae. We hypothesized that the decrease in dissolved oxygen due to microbes in the organic material could increase persistence of the microaerophilic trichomonads. Using simulated birdbaths, we determined 1) the levels of dissolved oxygen in distilled water with various amounts of organic material, 2...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Abstract Reptiles are becoming popular pets in many parts of the world. They are also known to harbor numerous gastrointestinal parasites. We used faecal smears to examine 748 stool samples from 14 different agamid lizard species. In addition, we used coproantigen ELISA tests (11 samples) and immunofluorescence assays (IFA) (19 samples) to detect reptile Cryptosporidium infections. In 28 cases, veterinarians requested therapy to treat oxyurid- and/or Isospora amphiboluri-infections and resent fecal samples after proposed therapy and anti-parasitic treatments had been applied. We also performed complete dissections...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Authors: Mendoza-Oliveros T, Arana-Argáez V, Alvaréz-Sánchez LC, Lara-Riegos J, Alvaréz-Sánchez ME, Torres-Romero JC Abstract Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTR...
Source: Korean Journal of Parasitology - Category: Parasitology Tags: Korean J Parasitol Source Type: research
Purpose: Trichomonas vaginalis (T. vaginalis) is the most common parasitic infection among nonviral, sexually transmitted diseases in the urogenital system. The incidence of the disease can vary according to the socio-economic status of the societies or different social groups. This research was performed on women who had vaginal complaints and who applied to Eskisehir Cancer Research Early Diagnosis and Screening Center (KETEM) and Eskisehir State Hospital Obstetrics and Gynecology Clinics.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 20.118 Source Type: research
Publication date: Available online 25 January 2019Source: Inorganic Chemistry CommunicationsAuthor(s): Prinessa Chellan, Tameryn Stringer, Ajit Shokar, Aaron Au, Christina Tam, Luisa W. Cheng, Gregory S. Smith, Kirkwood M. LandAbstractClinical cases of metronidazole resistant Trichomonas vaginalis infections have spurred interest in drug discovery against this protozoal parasite. We have carried out a structure-activity studies using mononuclear palladium(II) complexes containing salicylaldiminato thiosemicarbazones on a patient isolate of Trichomonas vaginalis highly resistant to the FDA-approved drug metronidazole. A sma...
Source: Inorganic Chemistry Communications - Category: Chemistry Source Type: research
Publication date: Available online 15 January 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Malek J. Hallinger, Anja Taubert, Carlos Hermosilla, Frank MutschmannAbstractReptiles are becoming popular pets in many parts of the world. They are also known to harbor numerous gastrointestinal parasites. We used faecal smears to examine 748 stool samples from 14 different agamid lizard species. In addition, we used coproantigen ELISA tests (11 samples) and immunofluorescence assays (IFA) (19 samples) to detect reptile Cryptosporidium infections. In 28 cases, veterinarians requested therapy to ...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
This study suggests that these analogues possess chemical features of interest to be further explored as alternatives for the treatment of trichomoniasis.Graphical abstract
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 26 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Torsten Wundenberg, Marcus M. Nalaskowski, Benjamin Löser, Werner Fanick, Thomas Hackl, Ursula Fürnkranz, Christoph Rehbach, Hongying Lin, Georg W. MayrAbstractThe parasitic protozoon Trichomonas vaginalis is the pathogen of trichomoniasis, the most common non-viral, sexually transmitted disease in humans. Inositol phosphates function in the pathomechanisms of a number of human pathogenic protozoa. Recent findings point to a role of inositol phosphates in T. vaginalis’ adaption to oxygen exposure duri...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research
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