Surface-dependent endocytosis of poly(isobutylcyanoacrylate) nanoparticles by Trichomonas vaginalis

Publication date: 5 September 2018Source: International Journal of Pharmaceutics, Volume 548, Issue 1Author(s): Sophia Malli, Christian Bories, Mickaël Bourge, Philippe.M. Loiseau, Kawthar BouchemalAbstractPrevious data from our research group showed that chitosan-coated poly(isobutylcyanoacrylate) nanoparticles (NPs) (denoted PIBCA/Chito20) exhibited intrinsic anti-Trichomonas vaginalis activity, while PIBCA/pluronic® F68 without chitosan (PIBCA/F68) were inactive. However, the mechanism of anti-T. vaginalis activity of chitosan-coated PIBCA NPs is still unknown. Our hypothesis is that chitosan-coated NPs are internalized by the parasite, contrarily to PIBCA/F68. In this investigation, the impact of NP surface on their internalization by the protozoan was studied using flow cytometry and parasite morphological changes after different incubation times with PIBCA/Chito20 NPs were monitored by electron microscopy. Flow-cytometry revealed that PIBCA/Chito20 NPs were uptaken by T. vaginalis as early as 10-min-incubation. Drastic cell morphological transformations were observed from scanning electron microscopy and transmission electron microscopy after incubation with PIBCA/Chito20 NPs. Numerous pits were seen on cell membrane since 10 min. Gradual increase in contact time increased NP endocytosis and induced proportional damages to T. vaginalis membrane. Then, investigation of whether PIBCA/Chito20 NPs can improve MTZ anti-T. vaginalis activity was studied using check...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 15 November 2018Source: International Journal of PharmaceuticsAuthor(s): Arash Yunessnia lehi, Hamidreza Shagholani, Ali Nikpay, Mohsen Ghorbani, Mohammad Soleimani lashkenari, Maryam SoltaniAbstractClinical resistance to drugs and diminution in their side effects have become great issues for pharmacologists. In veterinary medicine, parasites like Trichomonas gallinae are of veterinary, hygienic and economic importance and can be treated by metronidazole. Unfortunately, scientific evidence has been reported about its resistance and serious side effects in trichomoniasis. In this research,...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Trichomonas vaginalis is a sexually transmitted, eukaryotic parasite that causes trichomoniasis, the most common nonviral, sexually transmitted disease in the USA and worldwide. Little is known about the molecular mechanisms involved in the host immune response to this widespread parasite. Here we report thatT. vaginalis induces NLRP3 inflammasome activation in human macrophages, leading to caspase-1 activation and the processing of pro-IL-1 β to the mature and bioactive form of the cytokine. Using inhibitor-based approaches, we show that NLRP3 activation byT. vaginalis involves host cell detection of extracellular AT...
Source: Journal of Innate Immunity - Category: Allergy & Immunology Source Type: research
CONCLUSION: The present study suggests that manuka honey can be used as an additional therapy for the patient with giardiasis or trichomoniasis. However, in vivo study in the near future will elucidate more about the effectiveness of honey in treating parasitic infections.
Source: Indian Journal of Pharmacology - Category: Drugs & Pharmacology Authors: Source Type: research
In this study, the spectrum of urinary parasitic infections that had been reported in the last one and a half year was evaluated, and point prevalence in this zone was estimated. Microbiologist opinion had been taken in the difficult cases. Out of the total centrifuged urine sediments examined, urinary parasitic infection was found in 33 cases. The calculated point prevalence is 0.39%. Most common parasitic infection reported was flagellates (27 cases: 25T. vaginalis, 2 commensal flagellate closest toChylomastix), followed by three cases showing eggs ofEnterobius vermicularis, one case showing larvae ofStrongyloides sterco...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
(Natural News) A study conducted by the U.S. Department of Agriculture (DA) found that drinking more black tea will protect against trichomoniasis, one of the world’s most prevalent sexually transmitted diseases (STDs). This is due to protective compounds in the tea that can inhibit the parasite’s growth. Trichomoniasis is caused by the protozoan Trichomonas vaginalis. It renders people...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
CONCLUSIONS: It was proved that T. tenax, considered so far as a human specific parasite, can also inhabit the oral cavity of dog, cat and horse. To summarize, T. tenax was detected in the mouths of different domesticated animals, indicating that in Poland it can colonize a wider range of hosts than previously known. The owners of 3 dogs showed oral tissue inflammation of different intensity and were also positive for T. tenax; therefore, oral trichomonosis spread from humans to domestic animals and conversely should be taken into consideration. PMID: 30260189 [PubMed - in process]
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research
Authors: Thi Trung Thu T, Margarita V, Cocco AR, Marongiu A, Dessì D, Rappelli P, Fiori PL Abstract Mycoplasma hominis is considered an opportunistic pathogen able to colonize the lower urogenital tract; in females the infection is associated with severe pregnancy and postpartum complications, including abortion, endometritis, preterm delivery, and low birth weight. Molecular mechanisms of pathogenicity and virulence effectors remain poorly characterized. A number of studies in the last decade have demonstrated that M. hominis can establish an endosymbiotic relationship with Trichomonas vaginalis, a urogenit...
Source: Journal of Pregnancy - Category: OBGYN Tags: J Pregnancy Source Type: research
Abstract The parasite Trichomonas vaginalis (Tv) causes a highly prevalent sexually transmitted infection. As an extracellular pathogen, the parasite mediates adherence to epithelial cells to colonize the human host. In addition, the parasite interfaces with the host immune system and the vaginal microbiota. Modes of Tv pathogenesis include damage to host tissue mediated by parasite killing of host cells, disruption of steady-state vaginal microbial ecology, and eliciting inflammation by activating the host immune response. Recent Tv research has uncovered new players that contribute to multifactorial mechanisms o...
Source: Trends in Parasitology - Category: Parasitology Authors: Tags: Trends Parasitol Source Type: research
ConclusionsThese findings suggest that symbiosis with T. vaginalis has an enhancing effect on selected antimicrobial resistances of distinct M. hominis isolates.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
This study examines the inhibition promoted by Lactobacillus gasseri against the adhesion of T. vaginalis to host cells, a critical virulence aspect of this pathogen. We observed that the vaginal strain L. gasseri ATCC 9857 is highly inhibitory by various contact-dependent mechanisms and that surface proteins are largely responsible for this inhibitory phenotype. We found that the aggregation-promoting factor APF-2 from these bacteria significantly contributes to inhibition of the adhesion of T. vaginalis to human vaginal ectocervical cells. Understanding the molecules and mechanisms used by lactobacilli to protect the hos...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
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