Surface-dependent endocytosis of poly(isobutylcyanoacrylate) nanoparticles by Trichomonas vaginalis

Publication date: 5 September 2018Source: International Journal of Pharmaceutics, Volume 548, Issue 1Author(s): Sophia Malli, Christian Bories, Mickaël Bourge, Philippe.M. Loiseau, Kawthar BouchemalAbstractPrevious data from our research group showed that chitosan-coated poly(isobutylcyanoacrylate) nanoparticles (NPs) (denoted PIBCA/Chito20) exhibited intrinsic anti-Trichomonas vaginalis activity, while PIBCA/pluronic® F68 without chitosan (PIBCA/F68) were inactive. However, the mechanism of anti-T. vaginalis activity of chitosan-coated PIBCA NPs is still unknown. Our hypothesis is that chitosan-coated NPs are internalized by the parasite, contrarily to PIBCA/F68. In this investigation, the impact of NP surface on their internalization by the protozoan was studied using flow cytometry and parasite morphological changes after different incubation times with PIBCA/Chito20 NPs were monitored by electron microscopy. Flow-cytometry revealed that PIBCA/Chito20 NPs were uptaken by T. vaginalis as early as 10-min-incubation. Drastic cell morphological transformations were observed from scanning electron microscopy and transmission electron microscopy after incubation with PIBCA/Chito20 NPs. Numerous pits were seen on cell membrane since 10 min. Gradual increase in contact time increased NP endocytosis and induced proportional damages to T. vaginalis membrane. Then, investigation of whether PIBCA/Chito20 NPs can improve MTZ anti-T. vaginalis activity was studied using check...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Publication date: 10 November 2018Source: Sensors and Actuators B: Chemical, Volume 273Author(s): N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. HeliAbstractThe widespread bacteria, parasites and viruses are considered as the potential sources of infections which can endanger the public health. Accordingly, sensitive, rapid and cost-effective analysis methods are required for identification of the pathogens to achieve an improved quality of life. Trichomonas vaginalis is among the commonly colonizing parasite which threatens human health by causing Trichomoniasis infection. In the prese...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
In conclusion, our data demonstrate that individuals infected with trichomonas vaginalis have a higher risk of cervical cancer, especially co-infected with Human Papilloma Virus. Besides, there is significant regional and racial variation in the correlation between trichomonas vaginalis infection and risk of cervical cancer.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
Publication date: June–July 2018Source: Microbes and Infection, Volume 20, Issue 6Author(s): Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon ShinAbstractTrichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regu...
Source: Microbes and Infection - Category: Infectious Diseases Source Type: research
Publication date: Available online 30 June 2018Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Krysta H. Rogers, Yvette A. Girard, Leslie W. Woods, Christine K. JohnsonAbstractAvian trichomonosis is an upper digestive tract disease of birds typically caused by the protozoan parasite Trichomonas gallinae. In California (U.S.A), trichomonosis is known to cause periodic epidemics in the Pacific Coast band-tailed pigeon (Patagioenas fasciata monolis), a migratory upland game bird. We summarize the mortality events that occurred during winter 2014–2015 including the duration, estimated mor...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Publication date: 10 November 2018Source: Sensors and Actuators B: Chemical, Volume 273Author(s): N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. HeliAbstractThe widespread bacteria, parasites and viruses are considered as the potential sources of infections which can endanger the public health. Accordingly, sensitive, rapid and cost-effective analysis methods are required for identification of the pathogens to achieve an improved quality of life. Trichomonas vaginalis is among the commonly colonizing parasite which threatens human health by causing Trichomoniasis infection. In the prese...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
In conclusion, our data demonstrate that individuals infected with trichomonas vaginalis have a higher risk of cervical cancer, especially co-infected with Human Papilloma Virus. Besides, there is significant regional and racial variation in the correlation between trichomonas vaginalis infection and risk of cervical cancer.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
Publication date: Available online 31 May 2018Source: Microbes and InfectionAuthor(s): Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon ShinAbstractTrichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of int...
Source: Microbes and Infection - Category: Infectious Diseases Source Type: research
Publication date: Available online 30 June 2018Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Krysta H. Rogers, Yvette A. Girard, Leslie W. Woods, Christine K. JohnsonAbstractAvian trichomonosis is an upper digestive tract disease of birds typically caused by the protozoan parasite Trichomonas gallinae. In California (U.S.A), trichomonosis is known to cause periodic epidemics in the Pacific Coast band-tailed pigeon (Patagioenas fasciata monolis), a migratory upland game bird. We summarize the mortality events that occurred during winter 2014–2015 including the duration, estimated mor...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
In conclusion, our data demonstrate that individuals infected with trichomonas vaginalis have a higher risk of cervical cancer, especially co-infected with Human Papilloma Virus. Besides, there is significant regional and racial variation in the correlation between trichomonas vaginalis infection and risk of cervical cancer.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
Publication date: Available online 30 June 2018 Source:International Journal for Parasitology: Parasites and Wildlife Author(s): Krysta H. Rogers, Yvette A. Girard, Leslie W. Woods, Christine K. Johnson Avian trichomonosis is an upper digestive tract disease of birds typically caused by the protozoan parasite Trichomonas gallinae. In California (U.S.A), trichomonosis is known to cause periodic epidemics in the Pacific Coast band-tailed pigeon (Patagioenas fasciata monolis), a migratory upland game bird. We summarize the mortality events that occurred during winter 2014–2015 including the duration, estimated mortalit...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
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