Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells.

Conclusions: Robust optimization to increase the signal-to-noise ratio of immunofluorescent staining of bone marrow is required in order to achieve the necessary sensitivity and specificity for rare cell detection. Background immunofluorescent staining in bone marrow causes uncertainty and inconsistency among investigators, which can be overcome by systematically addressing each contributing source. Our optimized assay eliminates sources of background signal, and is adaptable to automated staining platforms for high throughput analysis.
PMID: 29988526 [PubMed]

Source: Biological Procedures Online - July 12, 2018 Category: Molecular Biology Authors: Axelrod HD, Pienta KJ, Valkenburg KC Tags: Biol Proced Online Source Type: research