Potential bactericidal activity of S. nux-vomica–ZnO nanocomposite against Multidrug-resistant bacterial pathogens and wound-healing properties

Publication date: Available online 11 July 2018Source: Journal of Trace Elements in Medicine and BiologyAuthor(s): Katherin Steffy, G. Shanthi, Anson. S. Maroky, S. SelvakumarAbstractMultidrug resistance in bacterial strains has become the greatest challenge for healthcare professionals for treating non-healing ulcers such as diabetic foot infections (DFI). Plant-mediated synthesis of S. nux-vomica–ZnO nanocomposite appears as a potential new alternative therapeutic agent that might be capable of tackling antibiotic-resistant bacterial pathogens and for treating a non-healing ulcer. The aim of the study was to investigate the antibacterial potential of S. nux-vomica–ZnO nanocomposite biosynthesised from Strychnos nux-vomica against multidrug-resistant organisms (MDROs) from DFU, wound-healing properties, and cytotoxic effects. The antibacterial potential was assessed by minimum inhibitory concentration (MIC)/ minimum bactericidal concentration (MBC) assays, time-kill kinetics, protein-leakage, and flow cytometric analysis. The wound-healing properties were assessed by scratch assay on mouse L929 fibroblastic cell line to quantify cell migration towards the injured area. Cytotoxicity was assessed using 3-[4,5-dimethyl-2-thiazol-yl]-2,5-diphenyl- 2H-tetrazolium bromide (MTT) cellular viability assay on the L929 cell line and human embryonic kidney epithelial (HEK-293) cell line. Strychnos nux-vomica–ZnO nanocomposite at a size range of 10–12 nm exhibited significant b...
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research