Development of functionalized gold nanoparticles as nanoflare probes for rapid detection of classical swine fever virus

Publication date: Available online 11 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Pengbo Ning, Zhongxing Wu, Xuepeng Li, Yulu Zhou, Aoxue Hu, Xiaocheng Gong, Jun He, Yuqiong Xia, Kangkang Guo, Ruili Zhang, Xianghan Zhang, Zhongliang WangAbstractClassical swine fever (CSF) is a devastating viral disease affecting pigs that causes major economic losses worldwide. Conventional assays to identify classical swine fever virus (CSFV) face challenges, such as the required molecular amplification of the target molecules via polymerase chain reaction (PCR). We designed a gold nanoflare probe to directly detect CSFV. Gold nanoparticles (AuNPs) were conjugated with a pair of complementary DNA sequences that specifically recognized and captured CSFV RNA, resulting in a fluorescence signal to indicate the existence of CSFV. The constructed nanocomposite was then utilized in a quantitative analysis to recognize the virus sequence present at amounts as low as 50 pg/μL. The CSFV-AuNP probe enabled real-time, quantitative detection of native CSFV in response to doses of the specific RNA sequence (CSFV NS2) that indicated active viral replication of CSFV Shimen in macrophages after 12, 24, and 48 h. The potential diagnostic applications of the probe were demonstrated by measuring CSFV without nucleic acid amplification in samples from seven types of tissue samples, specifically heart, spleen, kidney, liver, lymph, intestine, and muscle samples obtained from one pig co...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research