A new strategy for choosing “Q-markers” via network pharmacology, application to the quality control of a Chinese medical preparation

Publication date: April 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 2Author(s): Wei Xiang, Tong-Chuan Suo, Hua Yu, An-Ping Li, Shou-Qing Zhang, Chun-Hua Wang, Yan Zhu, Zheng LiAbstractDue to its chemical complexity, proper quality control for a Chinese medical preparation (CMP) has been a great challenge. Choosing the appropriate quality markers (Q-markers) for quality control of CMP is an important work. Best of all, the chosen Q-markers are the main chemical compounds from the herbals as well as the active constituents of this CMP. Only in this way the established quality control system can really achieve the purpose of controlling the quality of CMP and ensuring the safely and effectively use of CMP. To achieve the purpose, network pharmacology combined with the contents of chemical compounds in the CMP has been used in this research. We took an anti-arrhythmic CMP, Shenxian-Shengmai oral liquid (SSOL), as an example. Firstly, UPLC-QTOF-MS/MS method was used to analyze the main components of SSOL. A total of 64 compounds were unambiguously or tentatively identified and 32 of them were further validated by reference compounds. Secondly, the network was constructed based on the identified compounds to predict the effective compounds related to cardiac arrhythmias. Based on the existing database and the operation method of topology, a method of double network analysis (DNAA) was proposed, from which 10 important targets in the pathway of arrhythmia were sc...
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research