P-Rex1 is dispensable for Erk activation and mitogenesis in breast cancer.

P-Rex1 is dispensable for Erk activation and mitogenesis in breast cancer. Oncotarget. 2018 Jun 19;9(47):28612-28624 Authors: Barrio-Real L, Lopez-Haber C, Casado-Medrano V, Goglia AG, Toettcher JE, Caloca MJ, Kazanietz MG Abstract Phosphatidylinositol-3,4,5-Trisphosphate Dependent Rac Exchange Factor 1 (P-Rex1) is a key mediator of growth factor-induced activation of Rac1, a small GTP-binding protein widely implicated in actin cytoskeleton reorganization. This Guanine nucleotide Exchange Factor (GEF) is overexpressed in human luminal breast cancer, and its expression associates with disease progression, metastatic dissemination and poor outcome. Despite the established contribution of P-Rex1 to Rac activation and cell locomotion, whether this Rac-GEF has any relevant role in mitogenesis has been a subject of controversy. To tackle the discrepancies among various reports, we carried out an exhaustive analysis of the potential involvement of P-Rex1 on the activation of the mitogenic Erk pathway. Using a range of luminal breast cancer cellular models, we unequivocally showed that silencing P-Rex1 (transiently, stably, using multiple siRNA sequences) had no effect on the phospho-Erk response upon stimulation with growth factors (EGF, heregulin, IGF-I) or a GPCR ligand (SDF-1). The lack of involvement of P-Rex1 in Erk activation was confirmed at the single cell level using a fluorescent biosensor of Erk kinase activity. Depletion of P-Rex1 from breast cancer ...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Related Links:

AbstractBreast cancer is the most common cancer affecting women in South Africa. There is little knowledge of beliefs to help identify key areas to improve support and education in this demographically and culturally diverse population. Women with a variety of demographic and socioeconomic characteristics accessing care for breast cancer were asked their agreement to statements of knowledge and beliefs about breast cancer. Of the 259 participants, positive statements of medical cure (87.9%) and family support (90.5%) were most commonly believed. Beliefs in faith-based cure and alternative treatments were also present (79.5...
Source: Journal of Cancer Education - Category: Cancer & Oncology Source Type: research
AbstractThe aims of this study are to assess knowledge of inheritance characteristics of breast cancer and risk reduction strategies and to determine risk perception and the factors affecting risk perception of women with family history. There is a gap in our understanding of risk perception and knowledge of genetic aspect of breast cancer and risk reduction strategies in women with a family history of breast cancer. The study design is descriptive cross-sectional study. Between January 2015 and 2016 at a training and research hospital in Turkey, 117 women who were the first- and second-degree relatives of breast cancer pa...
Source: Journal of Cancer Education - Category: Cancer & Oncology Source Type: research
In this report, magnetic field enhancement of the photodynamic therapy (PDT) efficacy of a novel methoxypolyethyleneglycol-thiol-SPIONs-gold-meso-tetrakis(4-hydroxyphenyl)porphyrin conjugate (nano-drug) against MCF-7 breast cancer cells was evaluated. The nano-drug exhibited excellent blue and red emissions under suitable ultraviolet (380 nm) and visible (430 nm) excitations and was well taken up by the cells without any significant dark cytotoxicity after 24 h post-incubation. However, after exposure of cells to light for about 15 min, high rate of cell death was observed in a dose-dependent manner. In addition, t...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
In conclusion, HCT-B-DTX-PNPs were found to possess a higher affinity for breast cancer cells and induce a stronger cytotoxicity than that of other PNPs.Graphical abstract
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
CONCLUSIONS: Serum PSA could be a useful biomarker for the diagnosis of breast cancer, and a biomarker for the differential diagnosis of breast cancer from benign breast tumors. PMID: 30024607 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Publication date: Available online 15 September 2017Source: Inorganica Chimica ActaAuthor(s): Edward D. Cross, M. Trisha C. Ang, D. Douglas Richards, Amy C. Clemens, Harshiny Muthukumar, Robert McDonald, London Woodfolk, Karina Ckless, Matthias BierenstielAbstractNS-containing ligands with the 2-thioaniline motif and imine functional group linked phenyl, 2-pyridyl and 2-thienyl pendants were synthesized by imine condensation reactions with corresponding aldehydes. 2-(methylthio)-N-(2-thienylmethylene)-benzenamine is a new compound characterized by NMR, FTIR and UV–vis. Single crystal X-ray crystallographic data was o...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Researchers from America's Harvard School of Public Health found that women's risk of getting breast cancer could fall by 11 per cent if they eat more than five portions of fruit and veggies a day.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Publication date: Available online 20 July 2018Source: Biochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsAuthor(s): Jinlong Wang, Xiuyuan Zhang, Jiming Ling, Yun Wang, Xiaolin Xu, Yuchen Liu, Chaozhi Jin, Jiyu Ju, Yanzhi Yuan, Fuchu He, Chunling Zhao, Jian Wang, Chunyan TianAbstractKRAB-containing zinc finger proteins (KZNF) constitute the largest family of transcriptional regulators in humans and play critical roles in normal development and tumorigenesis. However, the function and mechanism of most KZNFs remain unclear. Here, we report that ZNF496, a KZNF family member, interacts with the DNA binding domain...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - Category: Genetics & Stem Cells Source Type: research
Condition:   Hormone Receptor Positive Malignant Neoplasm of Breast Interventions:   Behavioral: Episodic future thinking;   Behavioral: Episodic recent thinking Sponsors:   Virginia Polytechnic Institute and State University;   Carilion Clinic Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Breast Neoplasms Interventions:   Drug: Olaparib;   Drug: Durvalumab Sponsors:   Seoul National University Hospital;   AstraZeneca Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
More News: Breast Cancer | Cancer | Cancer & Oncology